Applied Biochemistry and Biotechnology

, Volume 124, Issue 1–3, pp 901–910 | Cite as

Enzyme recovery and recycling following hydrolysis of ammonia fiber explosion-treated corn stover

  • Bernie Steele
  • Srini Raj
  • John Nghiem
  • Mark Stowers


Both cellulase and cellobiase can be effectively recovered from hydrolyzed biomass using an ultrafiltration recovery method. Recovery of cellulase ranged from 60 to 66.6% and for cellobiase from 76.4 to 88%. Economic analysis shows that cost savings gained by enzyme recycling are sensitive to enzyme pricing and loading. At the demonstrated recovery of 60% and current loading of 15 Filter paper units of cellulase/g of glucan, enzyme recycling is expected to generate a cost savings of approx 15%. If recovery efficiency can be improved to 70%, the savings will increase to >25%, and at 90% recovery the savings will be 50%.

Index Entries

Enzyme recycle biomass ammonia fiber explosion ethanol corn stover 


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Copyright information

© Humana Press Inc. 2005

Authors and Affiliations

  • Bernie Steele
    • 1
  • Srini Raj
    • 1
  • John Nghiem
    • 1
  • Mark Stowers
    • 1
  1. 1.MBI InternationalLansing

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