Enzyme recovery and recycling following hydrolysis of ammonia fiber explosion-treated corn stover
Both cellulase and cellobiase can be effectively recovered from hydrolyzed biomass using an ultrafiltration recovery method. Recovery of cellulase ranged from 60 to 66.6% and for cellobiase from 76.4 to 88%. Economic analysis shows that cost savings gained by enzyme recycling are sensitive to enzyme pricing and loading. At the demonstrated recovery of 60% and current loading of 15 Filter paper units of cellulase/g of glucan, enzyme recycling is expected to generate a cost savings of approx 15%. If recovery efficiency can be improved to 70%, the savings will increase to >25%, and at 90% recovery the savings will be 50%.
Index EntriesEnzyme recycle biomass ammonia fiber explosion ethanol corn stover
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- 1.National Bioenergy Center Sugar Platform Integration Project. Quarterly Update. January/March 2004, #2, http://www.eere.energy.gov/biomass/pdfs/35605.pdf.Google Scholar
- 2.U.S. Department of Energy, Energy Efficiency and Renewable Energy. Cellulase Cost-Reduction Contracts. Last updated: May 23, 2004, http://www.eere.energy.gov/biomass/cellulase_cost.htmlGoogle Scholar
- 3.Dale, B. E. and Weaver, J. K. (2000), US patent 6,106,888.Google Scholar
- 4.Dale, B. E. (1986), US patent 4,600,590, pp. 1–7.Google Scholar
- 5.Wilke, C. R., Yang, R. D. and Stockar, U. V. (1976), Biotechnol. Bioeng. 19, 155.Google Scholar
- 6.Linko, M. (1977), in Advances in Biochemical Engineering, vol. 5, Ghose, T. K., Fiechter, A., and Blakebrough, N., eds., Springer-Verlag, New York, pp. 25–48.Google Scholar
- 11.Zaldivar, M., J.C. Velasquez, I. Contreras, and L.M. Perez. (2001), Electronic J. Biotechnol. (online), December 15, 2001, vol. 4, no. 3, http://www.ejb.org/content/vol4/issue3/full/7.Google Scholar