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Microbial synthesis and characterization of physiochemical properties of polyhydroxyalkanoates (PHAs) produced by bacteria isolated from activated sludge obtained from the municipal wastewater works in Hong Kong

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Abstract

The first objective of this study was the measurement of physical properties of P(3HB-co-3HV) copolymers with different (hydroxybutyrate) HB to (hydroxyvalerate) HV ratios produced by Bacillus cereus (TRY2) isolated from activated sludge. The 3HV PHBV copolymers were 0.05, 22.6, 39.2, 54.1, and 69.1 mol%, respectively. The second objective was to study possible wastewater treatment and production of PHAs at the same time by B. cereus (TRY2) and Pseudomonas spp. (TOB17) (both were isolated from activated sludge), recombinant Bacillus DH5α, and a combination of the above three bacteria. The results were satisfactory; the maximum COD and TOC of the sewage sludge reduced were 53.5% and 67.5%, respectively.

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Authors and Affiliations

  1. State Key Laboratory of Chinese Medicine and Molecular Pharmacology, Shenzen, China

    Ma Tsz-Chun, P. L. Chan, W. H. Lo & Peter Hoifu Yu

  2. Department of CSE, Hong Kong Polytechnic University, Hong Kong, China

    H. Chua

  3. Department of Biochemistry, University of Toronto, Ontario, Canada

    H. Lawford

  4. Open Laboratory of Chirotechnology of the Institute of Molecular Technology for Drug Discovery & Synthesis and the Department of Applied Biology and Chemical Technology, Hong Kong Polytechnic University, Hong Kong, China

    Ma Tsz-Chun, P. L. Chan, W. H. Lo & Peter Hoifu Yu

Authors
  1. Ma Tsz-Chun
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  2. P. L. Chan
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  3. H. Lawford
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  4. H. Chua
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  5. W. H. Lo
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  6. Peter Hoifu Yu
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Tsz-Chun, M., Chan, P.L., Lawford, H. et al. Microbial synthesis and characterization of physiochemical properties of polyhydroxyalkanoates (PHAs) produced by bacteria isolated from activated sludge obtained from the municipal wastewater works in Hong Kong. Appl Biochem Biotechnol 122, 731–739 (2005). https://doi.org/10.1385/ABAB:122:1-3:0731

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  • DOI: https://doi.org/10.1385/ABAB:122:1-3:0731

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