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Applied Biochemistry and Biotechnology

, Volume 122, Issue 1–3, pp 695–706 | Cite as

Ceriporiopsis subvermispora used in delignification of sugarcane bagasse prior to soda/anthraquinone pulping

  • Sirlene M. Costa
  • Adilson R. GonçalvesEmail author
  • Elisa Esposito
Article

Abstract

Sugarcane bagasse was pretreated with the white-rot fungus Ceriporiopsis subvermispora for 30 d of incubation. The solid-state fermentation of 800 g of bagasse was carried out in 20-L bioreactors with an inoculum charge of 250 mg of fungal mycelium/kg of bagasse. The oxidative enzymes manganese peroxidase (MnP), lignin peroxidase (LiP), and lac-case (Lac) and the hydrolytic enzyme xylanase (Xyl) were measured by standard methods and related to the fungus’s potential for delignification. Among the lignocellulolytic assayed enzymes, Xyl was detected in larger quantity (4478 IU/kg), followed by MnP (236 IU/kg). LiP and Lac were not detected. The results of chemical analysis and mass component loss showed that C. subvermispora was selective to lignin degradation. Pretreated sugarcane bagasse and control pulps were obtained by soda/anthraquinone (AQ) pulping. Pulp yields, kappa number, and viscosity of all pulps were determined by chemical analysis of the samples. Yields of soda/AQ ranged from 46 to 54%, kappa numbers were 15–25, and the viscosity ranged from 3.6 to 7 cP for pulps obtained from pretreated sugarcane bagasse.

Index Entries

Ceriporiopsis subvermispora sugarcane bagasse selective biodegradation hydrolytic enzymes oxidative enzymes soda/anthraquinone pulping lignin 

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Copyright information

© Humana Press Inc. 2005

Authors and Affiliations

  • Sirlene M. Costa
    • 1
  • Adilson R. Gonçalves
    • 1
    Email author
  • Elisa Esposito
    • 2
  1. 1.Departmento de BiotecnologiaFAENQUILLorena, SPBrazil
  2. 2.Núcleo Intergrado de BiotecnologiaUniversidade de Mogi das CruzesBrazil

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