Removing proteins from an aerated yeast fermentation by pulsing carbon dioxide
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Salting-out is a common technique used for precipitating proteins and other materials from fermentation and tissue culture processes. It leaves a salt residue in the system. Foam fractionation can also be used to remove proteins by protein precipitation from a dilute solution. In doing so, there is usually a trade-off between enrichment and recovery. An increase in the airflow rate will increase the recovery, but only at the expense of the enrichment. A new method for increasing the recovery in foam fractionations and in yeast fermentations is to add a burst of CO2 to the process and then restore the air. This CO2 acts like a temporary salt, but it does not leave behind a residue. The recovery increases as a result of the joint use of these gases, perhaps by more than 10-fold, without sacrificing the enrichment. Chicken egg albumin in a foam fractionation column can serve as a simple, experimental model for the proposed recovery process in lieu of the fermentation process.
Index EntriesSalting-out foam fractionation yeast fermentation protein concentration chicken egg albumin
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- 1.Eighth Symposium on Biotechnology for fuels and chemicals, C. D., Suitto, ed., John Willey and Sons, New York, pp. 633–643.Google Scholar
- 2.Effler, W. T. Jr., Tanner, R. D., and Malaney, G. W., (1989), in Bioproducts and Bioprocesses, A. Frichter, H. Ohada and R. D. Tanner, eds., Springer Verlag. Berlin. pp. 235–255.Google Scholar
- 3.Blanch, H. W. and Clark, D. S. (1997), in Biochemical Engineering, Marcel Dekker, New York, pp. 491–496.Google Scholar
- 4.Montero, G. A., Kirschner, T. F., and Tanner, R. D. (1993), Appl. Biochem. Biotechnol. 467–475.Google Scholar
- 6.Franklin, T. A., Malaney, G. W. and Tanner, R. D. (1986), J. Microbiol. Biotechnol. 1(2), 78–86.Google Scholar