Advertisement

Applied Biochemistry and Biotechnology

, Volume 122, Issue 1–3, pp 673–683 | Cite as

Sugarcane bagasse as raw material and immobilization support for xylitol production

  • Júlio C. Santos
  • Ícaro R. G. Pinto
  • Walter Carvalho
  • Ismael M. Mancilha
  • Maria G. A. Felipe
  • Silvio S. SilvaEmail author
Article

Abstract

Xylose-to-xylitol bioconversion was performed utilizing Candida guillier-mondii immobilized in sugarcane bagasse and cultured in Erlenmeyer flasks using sugarcane bagasse hydrolysate as the source of xylose. Fermentations were carried out according to a factorial design, and the independent variables considered were treatment, average diameter, and amount of bagasse used as support for cell immobilization. By increasing the amount of support, the xylitol yield decreased, whereas the biomass yield increased. The diameter of the support did not influence xylitol production, and treatment of the bagasse with hexamethylene diamine prior to fermentation resulted in the highest amount of immobilized cells.

Index Entries

Xylitol cell immobilization sugarcane bagasse Candida guilliermondii hemicellulosic hydrolysate 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Orlando, U. S., Baes, A. U., Nishijima, W., and Okada, M. A. (2002), Bioresou. Technol. 83, 195–198.CrossRefGoogle Scholar
  2. 2.
    Pandey, A., Socool, C. R., Nigam, P., and Socool, V. (2000), Bioresou. Technol. 74, 69–80.CrossRefGoogle Scholar
  3. 3.
    Bär, A. (1991), in Alternative Sweeteners, L. O’Brien, and C. Gelardi, (eds.), Marcel Deckker, Inc., New York, pp. 349–379.Google Scholar
  4. 4.
    Alanen, P., Holsti, M. L., and Pienihakkinen, K. (2000), Acta Odontol. Scand. 58, 279–284.PubMedCrossRefGoogle Scholar
  5. 5.
    Uhari, M., Tapiainen, T., and Kontiokari, T. (2000), Vaccine 19 (suppl. 1), S144-S147.PubMedCrossRefGoogle Scholar
  6. 6.
    Roberto, I. C., Felipe, M. G. A., Mancilha, I. M., Vitolo, M., Sato S., and Silva, S. S. (1995), Bioresou. Technol. 51, 255–257.CrossRefGoogle Scholar
  7. 7.
    Winkelhausen, E. and Kusmanova, S. (1998), J. Ferment. Bioeng. 86, 1–14.CrossRefGoogle Scholar
  8. 8.
    Silva, C. J. S. M. and Roberto, I. C. (2001), Process Biochem. 36, 1119–1124.CrossRefGoogle Scholar
  9. 9.
    Roseiro, J. C., Peito, M. A., Gírio, F. M., and Amarlal-Collaço, M. T. (1991), Arch. Microbiol. 156, 484–490.Google Scholar
  10. 10.
    Silva, S. S., Ribeiro, J. D., Felipe, M. G. A., and Vitolo, M. (1997), Appl. Biochem. Biotechnol. 63/64, 557–563.Google Scholar
  11. 11.
    Carvalho, W., Silva, S. S., Vitolo, M., and Mancilha, I. M. (2000), Zeitschrift Naturforschung 55c, 213–217.Google Scholar
  12. 12.
    Carvalho, W., Silva, S. S., Santos, J. C., and Converti, A. (2003), Enzyme Microb. Technol. 32, 553–559.CrossRefGoogle Scholar
  13. 13.
    Santos, J. C., Carvalho, W., Silva, S. S., and Converti, A. (2003), Biotechnol. Prog. 19, 1210–1215.PubMedCrossRefGoogle Scholar
  14. 14.
    Corcoran, E. (1985), in Topics in Enzyme and Fermentation Biotechnology, Ellis Horwood, Chichester, UK, vol. 10, pp. 12–50.Google Scholar
  15. 15.
    Brodelius, P. and Vandamme, E. J. (1987), in Biotechnology—A Comprehensive Treatise, vol. 7, Verlag Chemie, Weinheim, Germany, pp. 405–464.Google Scholar
  16. 16.
    Dervakos, G. A. and Webb, C. (1991), Biotechnol. Advertisements 9, 559–612.CrossRefGoogle Scholar
  17. 17.
    Akin, C. (1987), Biotechnol. Genet. Eng. Rev. 5, 319–367.PubMedGoogle Scholar
  18. 18.
    Alves, L. A., Felipe, M. G. A., Silva, J. B. A., Silva, S. S., and Prata, A. M. R. (1998), Appl. Biochem. Biotechnol. 70/72, 89–98.CrossRefGoogle Scholar
  19. 19.
    Barbosa, M. F. S., Medeiros, M. B., Mancilha, I. M., Scheneider, H., and Lee, H. (1988), J. Ind. Microbiol. 3, 241–251.CrossRefGoogle Scholar
  20. 20.
    Tyagi, R. D., Gupta, S. K., and Chand, S. (1992), Process Biochem. 27, 23–32.CrossRefGoogle Scholar
  21. 21.
    Porath, J. and Fornstedt, N. (1970), J. Chromatogr. 51, 497–489.CrossRefGoogle Scholar
  22. 22.
    Santos, J. C., Converti, A., Carvalho, W., Mussatto, S. I., and Silva, S. S. (2005), Process Biochem. 40, 113–118.CrossRefGoogle Scholar
  23. 23.
    Mussatto, S. I. and Roberto, I. C. (2003), J. Appl. Microbiol. 95, 331–337.PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press Inc. 2005

Authors and Affiliations

  • Júlio C. Santos
    • 1
  • Ícaro R. G. Pinto
    • 1
  • Walter Carvalho
    • 1
  • Ismael M. Mancilha
    • 1
  • Maria G. A. Felipe
    • 1
  • Silvio S. Silva
    • 1
    Email author
  1. 1.Departamento de BiotecnologiaFaculdade de Engenharia Química de LorenaLorena/SPBrazil

Personalised recommendations