Applied Biochemistry and Biotechnology

, Volume 122, Issue 1–3, pp 575–580 | Cite as

Effects of fatty acids on growth and poly-3-hydroxybutyrate production in bacteria

  • K. W. Lo
  • H. Chua
  • H. Lawford
  • W. H. Lo
  • Peter H. F. Yu


The effects of saturated and unsaturated fatty acids (lauric acid, palmitic acid, steric acid, oleic acid, linoleic acid, soybean oil) on Sphaerotilus natans, 0B17 (Pseudomonas sp.), and recombinant Escherichia coli DH5(/pUC19/CAB were studied. Oleic acid enhances Poly-3-hydroxybutyrate (PHB) production in these three bacterial strains, suggesting that the single double bond of the acid activates the polyhydroxylkanoate accumulation enzymatic reaction. Under the effect of lauric acid and linoleic acid, the growth of S. natans and 0B17 were totally inhibited. However, the enhanced PHB accumulation in recombinant E. coli was observed.

Index Entries

Fatty acids poly-3-hydroxybutyrate Sphaerotilus natans recombinant Escherichia coli Pseudomonas sp. 


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Copyright information

© Humana Press Inc. 2005

Authors and Affiliations

  • K. W. Lo
    • 1
    • 2
  • H. Chua
    • 3
  • H. Lawford
    • 4
  • W. H. Lo
    • 3
  • Peter H. F. Yu
    • 3
  1. 1.State Key Laboratory of Chinese Medicine and Molecular PharmacologyShenzenChina
  2. 2.Open Laboratory of Chirotechnology of the Institute of Molecular Technology for Drug Discovery & Synthesis and the Dept. of Applied Biology and Chemical TechnologyHong Kong Polytechnic UniversityHong KongChina
  3. 3.Department of CSEHong Kong Polytechnic UniversityHong KongChina
  4. 4.Dept. of BiochemistryUniversity of TorontoOntarioCanada

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