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Applied Biochemistry and Biotechnology

, Volume 122, Issue 1–3, pp 561–573 | Cite as

Cellulase production by Trichoderma reesei using sawdust hydrolysate

  • Chi-Ming Lo
  • Qin Zhang
  • Patrick Lee
  • Lu-Kwang JuEmail author
Article

Abstract

Sawdust hydrolysates were investigated for their ability to support cell growth and cellulase production, and for potential inhibition of Trichoderma reesei Rut C30. Simultaneous fermentations were conducted to compare the hydrolysate-based media with the controls having equivalent concentrations of glucose and Avicel cellulose. Six hydrolysates differing in the boiling durations in the hydrolysis procedure were evaluated. The hydrolysates were found to support cell growth and induce active cellulase synthesis. The maximum specific cellulase production rate was 0.046 filter paper units (FPU)/(g of cells · h) in the hydrolysate-based systems, much higher than that (0.017 FPU/[g of cells · h]) in the controls.

Index Entries

Acid hydrolysis cellulase induction inhibition Trichoderma reesei 

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Copyright information

© Humana Press Inc. 2005

Authors and Affiliations

  • Chi-Ming Lo
    • 1
  • Qin Zhang
    • 1
  • Patrick Lee
    • 2
  • Lu-Kwang Ju
    • 1
    Email author
  1. 1.Department of Chemical EngineeringThe University of AkronAkron
  2. 2.Tennessee Valley Authority, Public Power InstituteMuscle Shoals

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