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Applied Biochemistry and Biotechnology

, Volume 121, Issue 1–3, pp 427–437 | Cite as

Evaluation of inoculum of Candida guilliermondii grown in presence of glucose on xylose reductase and xylitol dehydrogenase activities and xylitol production during batch fermentation of sugarcane bagasse hydrolysate

  • Débora Danielle Virgínio da Silva
  • Maria das Graças de Almeida Felipe
  • Ismael Maciel de Mancilha
  • Sílvio Silvério da Silva
Article

Abstract

The effect of glucose on xylose-xylitol metabolism in fermentation medium consisting of sugarcane bagasse hydrolysate was evaluated by employing an inoculum of Candida guilliermondii grown in synthetic media containing, as carbon sources, glucose (30 g/L), xylose (30 g/L), or a mixture of glucose (2 g/L) and xylose (30 g/L). The inoculum medium containing glucose promoted a 2.5-fold increase in xylose reductase activity (0.582 IU/mgprot) and a 2-fold increase in xylitol dehydrogenase activity (0.203 IU/mgprot) when compared with an inoculum-grown medium containing only xylose. The improvement in enzyme activities resulted in higher values of xylitol yield (0.56 g/g) and productivity (0.46 g/[L·h]) after 48 h of fermentation.

Index Entries

Xylose reductase xylitol dehydrogenase glucose Candida guilliermondii sugarcane bagasse hydrolysate 

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Copyright information

© Humana Press Inc. 2005

Authors and Affiliations

  • Débora Danielle Virgínio da Silva
    • 1
  • Maria das Graças de Almeida Felipe
    • 1
  • Ismael Maciel de Mancilha
    • 1
    • 2
  • Sílvio Silvério da Silva
    • 1
  1. 1.Department of Biotechnology, Faculty of Chemical Engineering of LorenaFAENQUILLorena, SPBrazil
  2. 2.Department of Food TechnologyUFVViçosa, MGBrazil

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