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Lipase production by solid-state fermentation

Cultivation conditions and operation of tray and packed-bed bioreactors

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Abstract

The production of lipase by Penicillium simplicissimum in solid-state fermentation was studied using babassu cake as the basal medium. Tray-type and packed-bed bioreactors were employed. In the former, the influence of temperature; content of the medium, and medium supplementation with olive oil, sugarcane molasses, corn steep liquor, and yeast hydrolysate was studied. For all combinations of supplements, a temperature of 30°C, a moisture content of 70%, and a concentration of carbon source of 6.25% (m/m, dry basis) provided optimum conditions for lipase production. When used as single supplements olive oil and molasses also were able to provide high lipase activities (20 U/g). Using packed-bed bioreactors and molasses-supplemented medium, optimum conditions for enzyme production were air superficial velocities above 55 cm/min and temperatures below 28°C. The lower temperature optimum found for these reactors is probably related to radial heat gradient formation inside the packed bed. Maximum lipase activities obtained in these bioreactors (26.4 U/g) were 30% higher than in tray-type reactors.

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Authors and Affiliations

  1. Department of Biochemistry, Institute of Chemistry, Brazil

    Melissa L. E. Gutarra, Elisa D. C. Cavalcanti & Denise M. G. Freire

  2. COPPE, Chemical Engineering Program, Federal University of Rio de Janeiro, Centro de Tecnologia (CT), Laboratory 549-2, Ilha do Fundão, CEP, 21945-970, Rio de Janeiro/RJ, Brazil

    Leda R. Castilho & Geraldo L. Sant’Anna Jr.

Authors
  1. Melissa L. E. Gutarra
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  2. Elisa D. C. Cavalcanti
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  3. Leda R. Castilho
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  4. Denise M. G. Freire
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  5. Geraldo L. Sant’Anna Jr.
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Correspondence to Denise M. G. Freire.

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Gutarra, M.L.E., Cavalcanti, E.D.C., Castilho, L.R. et al. Lipase production by solid-state fermentation. Appl Biochem Biotechnol 121, 105–116 (2005). https://doi.org/10.1385/ABAB:121:1-3:0105

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  • DOI: https://doi.org/10.1385/ABAB:121:1-3:0105

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