Applied Biochemistry and Biotechnology

, Volume 119, Issue 2, pp 181–193 | Cite as

Effect of expression of manganese superoxide dismutase in baculovirus-infected insect cells

  • Ying Wang
  • Larry W. Oberley
  • Dale Howe
  • Donald L. Jarvis
  • Gaurav Chauhan
  • David W. MurhammerEmail author
Original Articles


It has previously been demonstrated that baculovirus infection of the Spodoptera frugiperda Sf-9 (Sf-9) and Trichoplusia ni BTI-Tn-5B1-4 (Tn-5B1-4) insect cell lines leads to oxidative stress as measured by protein and membrane lipid oxidation and that this oxidative damage contributes to cell death. As a result of these findings, it was hypothesized that baculovirus infection stimulates superoxide radical (O 2 ·— synthesis in the mitochondria and that the resulting O 2 ·— accumulation overwhelms the cells’ antioxidant defenses. We investigated the ability of manganese superoxide dismutase (MnSOD) expression (which reduces O 2 ·— to H2O2) to overcome the oxidative damage caused by baculovirus infection. It was found that MnSOD expression significantly reduced oxidative damage in baculovirus-infected Tn-5B1-4 cells but had no significant effect on oxidative damage in baculovirus-infected Sf-9 cells. The results are consistent with the hypothesis that O 2 ·— accumulation in the mitochondria is at least partially responsible for the oxidative damage resulting from the baculovirus infection of insect cells.

Index Entries

Spodoptera frugiperda Sf-9 Trichoplusia ni BTI-Tn-5B1-4 baculovirus expression vector system manganese superoxide dismutase catalase protein oxidation lipid oxidation 


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Copyright information

© Humana Press Inc. 2004

Authors and Affiliations

  • Ying Wang
    • 1
  • Larry W. Oberley
    • 2
  • Dale Howe
    • 3
  • Donald L. Jarvis
    • 3
  • Gaurav Chauhan
    • 1
  • David W. Murhammer
    • 1
    Email author
  1. 1.Department of Chemical and Biochemical EngineeringThe University of IowaIowa, City
  2. 2.Free Radical and Radiation Biology ProgramThe University of IowaIowa City
  3. 3.Department of Molecular BiologyUniversity of WyomingLaramie

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