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Xylanase production by the thermophilic mold Humicola lanuginosa in solid-state fermentation

  • Pankaj Kamra
  • T. SatyanarayanaEmail author
Original Articles

Abstract

Among the lignocellulosic substrates tested, wheat bran supported a high xylanase (EC 3.2.1.8) secretion by Humicola lanuginosa in solid-state fermentation (SSF). Enzyme production reached a peak in 72 h followed by a decline thereafter. Enzyme production was very high (7832 U/g of dry moldy bran) when wheat bran was moistened with tap water at a substrate-to-moistening agent ratio of 1:2.5 (w/v) and an inoculum level of 3 × 106 spores/10 g of wheat bran at a water activity (a w ) of 0.95. Cultivation of the mold in large enamel trays yielded a xylanase titer comparable with that in flasks. Parametric optimization resulted in a 31% increase in enzyme production in SSF. Xylanase production was approx 23-fold higher in SSF than in submerged fermentation (SmF). A threshold constitutive level of xylanase was secreted by H. lanuginosa in a medium containing glucose as the sole carbon source. The enzyme was induced by xylose and xylan. Enzyme synthesis was repressed beyond 1.0% (w/v) xylose in SmF, whereas it was unaffected up to 3.0% (w/w) in SSF, suggesting a minimization of catabolite repression in SSF.

Index Entries

Humicola lanuginosa solid-state fermentation submerged fermentation wheat bran xylanase 

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Copyright information

© Humana Press Inc. 2004

Authors and Affiliations

  1. 1.Department of MicrobiologyUniversity of Delhi South CampusNew DelhiIndia

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