Applied Biochemistry and Biotechnology

, Volume 119, Issue 2, pp 121–131 | Cite as

Immobilization of glucoamylase onto novel porous polymer supports of vinylene carbonate and 2-hydroxyethyl methacrylate

  • Yanli HuoEmail author
  • Yue Li
  • Zhi Yuan
  • Jiaxian Huang
Original Articles


Glucoamylase was immobilized onto novel porous polymer supports. The properties of immobilized glucoamylase and the relationship between the activity of immobilized enzyme and the properties of porous polymer supports were investigated. Compared with the native enzyme, the temperature profile of immobilized glucoamylase was widened, and the optimum pH was also changed. The optimum substrate concentration of immobilized glucoamylase was higher than that of native enzyme. After storage for 23 d, the immobilized glucoamylase still maintained about 84% of its initial activity, whereas the native enzyme only maintained about 58% of the initial activity. Moreover, after using repeatedly seven times, the immobilized enzyme maintained about 85% of its initial activity. Furthermore, the properties of porous polymer supports had an effect on the activity of the immobilized glucoamylase.

Index Entries

Glucoamylase immobilization porous supports vinylene carbonate 2-hydroxyethyl methacrylate 


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  1. 1.
    Fidel, T., Norman, B. J., and George, T. T. (1992), Prog. Biochem. 27, 177–181.CrossRefGoogle Scholar
  2. 2.
    Freire, D. M. G. and Santanna, J. G. L. (1990), Appl. Biochem. Biotechnol. 30, 23–34.Google Scholar
  3. 3.
    Jayaraju, K., Ayyanna, C., Padmasree, D. V. S., Subbarao, S., and Jaganadha Rao, R. (1991), Indian Chem. Eng. 33, 47–50.Google Scholar
  4. 4.
    Salam, R. B. P. and Venkatrem, T. (1989), Indian Chem. Eng. 31, 49–70.Google Scholar
  5. 5.
    Anna, W., Jersy, L., and Teresa, B. (1990), J. Chem. Tech. Biotechnol. 48, 287–301.Google Scholar
  6. 6.
    Bernard, R. P. and Gilbert, B. (1992), Enzyme Microb. Technol. 14, 361–370.CrossRefGoogle Scholar
  7. 7.
    Kazuhiro, H., Masayuki, T., Masafumi, K., and Michihiro, F. (1992), J. Chem. Eng. Japan 25, 569–574.CrossRefGoogle Scholar
  8. 8.
    Pieters, B. R., Bardeletti, G., and Coulet, P. R. (1992), Appl. Biochem. Biotechnol. 32, 37–53.PubMedCrossRefGoogle Scholar
  9. 9.
    Story, K. B., Duncan, J. A., and Chakrabarti, A. C. (1990), Appl. Biochem. Biotechnol. 23, 221–236.CrossRefGoogle Scholar
  10. 10.
    Zhou, H., Kong, W., Cha, X., Li, W., and Shen, J. C. (1992), J. Chem. Tech. Biotechnol. 54, 43–46.Google Scholar
  11. 11.
    Arica, M. Y. (2000), J. Appl. Polym. Sci. 77, 2000–2008.CrossRefGoogle Scholar
  12. 12.
    Arica, M. Y., Alaeddinoglu, N. G., and Hasirci, V. (1998), Enzyme Microb. Technol. 22, 152–157.CrossRefGoogle Scholar
  13. 13.
    Arica, M. Y., Yavuz, H., and Denizli, A. (2001), J. Appl. Polym. Sci. 81, 2702–2710.CrossRefGoogle Scholar
  14. 14.
    Arica, M. Y., Yavuz, H., Patir, S., and Denizli, A. (2000), J. Mol. Catal. B Enzymatic 11, 127–138.CrossRefGoogle Scholar
  15. 15.
    Bahar, T. and Celebi, S. S. (1998), Enzyme Microb. Technol. 23, 301–304.CrossRefGoogle Scholar
  16. 16.
    Bahar, T. and Celebi, S. S. (1999), J. Appl. Polym. Sci. 72, 69–73.CrossRefGoogle Scholar
  17. 17.
    Bahar, T. and Celebi, S. S. (2000), Enzyme Microb. Technol. 26, 28–33.CrossRefGoogle Scholar
  18. 18.
    Ding, L. H., Jiang, Y., Huang, L., Li, Y. G., and Huang, J. X. (2001), Appl. Biochem. Biotechnol. 95, 11–21.PubMedCrossRefGoogle Scholar
  19. 19.
    Ding, L. H., Li, Y., Jiang, Y., Cao, Z., and Huang, J. X. (2002), J. Appl. Polym. Sci. 83, 94–102.CrossRefGoogle Scholar
  20. 20.
    Ding, L. H. and Qu, B. J. (2001), React. Funct. Polym. 49, 67–76.CrossRefGoogle Scholar
  21. 21.
    Huang, J. X., Ding, L. H., Cao, Z., Li, Y., and Jiang, Y. (2001), Chem. J. Chin. Univ. Chinese 22, 678–682.Google Scholar
  22. 22.
    Huang, J. X., Huo, Y. L., Li, H., Yan, S. X., Sun, J. X., and Yuan, Z. (2001), Chin. J. Biochem. Mol. Biol. 17, 376–380.Google Scholar
  23. 23.
    Huo, Y. L., Li, Y., and Huang, J. X. (2001), Acta Scientiarum Naturalium Universitatis Nankaiensis 34, 1–5.Google Scholar
  24. 24.
    Miller, G. L. (1959), Anal. Chem. 31, 426–429.CrossRefGoogle Scholar

Copyright information

© Humana Press Inc. 2004

Authors and Affiliations

  1. 1.Department of Chemistry, The State Key Laboratory of Functional Polymer Materials for Adsorption and SeparationNankai UniversityTianjinP.R. China

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