Identification of microbial inhibitory functional groups in corn stover hydrolysate by carbon-13 nuclear magnetic resonance spectroscopy
- 162 Downloads
Dilute-acid biomass hydrolysates contain biomass degradation products that are inhibitory to cell growth and fermentation. Overliming with Ca(OH)2 has been found to be one of the most effective methods for detoxifying the dilute-acid hydrolysate for ethanol production. However, the mechanism of overliming is not well understood. Carbon-13 nuclear magnetic resonance (13C-NMR) spectroscopy was used to elucidate the functional groups involved in the overliming reaction. The 13C-NMR spectra showed that the major functional groups removed during the overliming process were aliphatic and aromatic acids or esters, and other aromatic and aliphatic compounds. Ketone and aldehyde functionalities were not detected in the spectra. This is the first time that 13C-NMR has been used to elucidate the overliming reaction.
Index EntriesOverliming inhibition corn stover hydrolysate carbon-13 nuclear magnetic resonance acetone
Unable to display preview. Download preview PDF.
- 1.The Technology Roadmaps for Plant/Crop-Based Renewable Resources 2020. DOE/GO-10099-706, February 1999. http://www.oit.doe.gov/agriculture.Google Scholar
- 2.Committee on Biobased Industrial Products. (1999), Biobased Industrial Products, Priorities for Research and Commercialization, National Academy Press, Washington, DC.Google Scholar
- 3.Hettenhaus, J., Wooley, R., and Ashworth, J. (2002), Final Report, National Renewable Energy Laboratory, contract no. ACO-1-31108-01, Golden, CO.Google Scholar
- 5.McMillan, J. D. (1994), in Enzymatic Conversion of Biomass for Fuels Production, ACS symposium series 566, Himmel, M. E., Baker, J. O., and Overend, R. P., eds., American Chemical Society, Washington, DC, Chapter 21.Google Scholar
- 18.Buhner, J. (2001), MS thesis, Technical University of Dresden, Germany.Google Scholar
- 19.Scalbert, A., Monties, B., Guittet, E., and Lallemand, J. Y. (1986), Holzforschung 40, 119–127.Google Scholar
- 21.McElroy, R. D. and Lai, K. (1988), J. Wood Chem. Technol. 8(3), 361–378.Google Scholar