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Influence of carbon and nitrogen sources and temperature on hyperproduction of a thermotolerant β-glucosidase from synthetic medium by Kluyveromyces marxianus

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Abstract

The effect of carbon source and its concentration, inoculum size, yeast extract concentration, nitrogen source, pH of the fermentation medium, and fermentation temperature on β-glucosidase production by Kluyveromyces marxianus in shake-flask culture was investigated. These were the independent variables that directly regulated the specific growth and β-glucosidase production rate. The highest product yield, specific product yield, and productivity of β-glucosidase occurred in the medium (pH 5.5) inoculated with 10% (v/v) inoculum of the culture. Cellobiose (20 g/L) significantly improved β-glucosidase production measured as product yield (Y P/S ) and volumetric productivity (Q P ) followed by sucrose, lactose, and xylose. The highest levels of productivity (144 IU/[L·h]) of β-glucosidase occurred on cellobiose in the presence of CSL at 35°C and are significantly higher than the values reported by other researchers on almost all other organisms. The thermodynamics and kinetics of β-glucosidase production and its deactivation are also reported. The enzyme was substantially stable at 60°C and may find application in some industrial processes.

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Abbreviations

h :

Planck’s constant

k B :

Boltzmann constant

q p :

specific rate of enzyme production (IU/[g of cells·h])

q S :

specific rate of substrate consumption (g of substrate consumed/[g of cells·h])

Q IP :

rate of intracellular protein formation (mg/[L·h])

Q P :

rate of β-glucosidase formation (IU/[L·h])

Q S :

rate of substrate consumption (g of substrate consumed/[L·h])

Q X :

rate of cell mass formation (g of cells/[L·h])

R :

gas constant

T :

absolute temperature

Y P/S :

β-glucosidase yield (IU/g of substrate utilized)

Y P/X :

specific yield of enzyme production (IU/g of cells)

Y X/S :

cell yield coefficient (g of cells formed/g of substrate utilized)

ΔH*:

enthalpy of activation

ΔS*:

entropy of activation

μ:

specific growth rate (h−1)

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Authors and Affiliations

  1. National Institute for Biotechnology and Genetic Engineering (NIBGE), PO Box 577, Faisalabad, Pakistan

    M. I. Rajoka, Samia Khan, Farooq Latif & Riaz Shahid

Authors
  1. M. I. Rajoka
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  2. Samia Khan
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  3. Farooq Latif
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  4. Riaz Shahid
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Correspondence to M. I. Rajoka.

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Rajoka, M.I., Khan, S., Latif, F. et al. Influence of carbon and nitrogen sources and temperature on hyperproduction of a thermotolerant β-glucosidase from synthetic medium by Kluyveromyces marxianus . Appl Biochem Biotechnol 117, 75–92 (2004). https://doi.org/10.1385/ABAB:117:2:075

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