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Evaluation of wet oxidation pretreatment for enzymatic hydrolysis of softwood

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Abstract

The wet oxidation pretreatment (water, oxygen, elevated temperature, and pressure) of softwood (Picea abies) was investigated for enhancing enzymatic hydrolysis. The pretreatment was preliminarily optimized. Six different combinations of reaction time, temperature, and pH were applied, and the compositions of solid and liquid fractions were analyzed. The solid fraction after wet oxidation contained 58–64% cellulose, 2–16% hemicellulose, and 24–30% lignin. The pretreatment series gave information about the roles of lignin and hemicellulose in the enzymatic hydrolysis. The temperature of the pretreatment, the residual hemicellulose content of the substrate, and the type of the commercial cellulase preparation used were the most important factors affecting the enzymatic hydrolysis. The highest sugar yield in a 72-h hydrolysis, 79% of theoretical, was obtained using a pretreatment of 200°C for 10 min at neutral pH.

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References

  1. Grethlein, H. E., Allen, D. C., and Converse, A. O. (1984), Biotechnol. Bioeng. 26, 1498–1505.

    Article  CAS  Google Scholar 

  2. Maekawa, E. (1996), Wood Sci. Technol. 30, 133–139.

    Article  CAS  MathSciNet  Google Scholar 

  3. Ramos, L. P., Breil, C., and Saddler, J. N. (1992), Appl. Biochem. Biotechnol. 34/35, 37–47.

    Google Scholar 

  4. Sjöström, E. (1993), Wood Chemistry: Fundamentals and Applications, Academic, London.

    Google Scholar 

  5. Mansfield, S. H., Mooney, C., and Saddler, J. N. (1999), Biotechnol. Prog. 15, 804–816.

    Article  PubMed  CAS  Google Scholar 

  6. Mooney, C. A., Mansfield, S. H., Touhy, M. G., and Saddler, J. N. (1998), Bioresour. Technol. 64, 113–119.

    Article  CAS  Google Scholar 

  7. Sutcliffe, R. and Saddler, J. N. (1986), Biotechnol. Bioeng. Symp. 17, 749.

    CAS  Google Scholar 

  8. Fengel, D. and Wegener, G. (1984), Wood: Chemistry, Ultrastructure, Reactions, de Gruyter, Berlin.

    Google Scholar 

  9. Hsu, T.-A. (1996), in Handbook of Bioethanol: Production and Utilization, Wyman, C. E., ed., Taylor & Francis, Washington, DC, pp. 179–212.

    Google Scholar 

  10. Bouchard, J., Nguyen, T. S., Chornet, E., and Overend, R. P. (1991), Bioresour. Technol. 26, 121–131.

    Article  Google Scholar 

  11. Clark, T. A. and Mackie, K. L. (1987), J. Wood Chem. Technol. 7, 373–403.

    CAS  Google Scholar 

  12. Palmqvist, E., Hahn-Hägerdal, B., Galbe, M., and Zacchi, G. (1996), Enzyme Microb. Technol. 19, 470–476.

    Article  CAS  Google Scholar 

  13. Larsson, S., Palmqvist, E., Hahn-Hägerdal, B., Tengborg, C., Stenberg, K., Zacchi, G., and Nilvebrant, N.-O. (1999), Enzyme Microb. Technol. 24, 151–159.

    Article  CAS  Google Scholar 

  14. McGinnis, G. D., Wilson, W. W., and Muller, C. E. (1983), Ind. Chem. Prod. Res. Dev. 22, 352–357.

    Article  CAS  Google Scholar 

  15. Taylor, J. E. and Weygandt, J. C. (1974), Can. J. Chem. 52, 1925–1933.

    Article  CAS  Google Scholar 

  16. Debellefontaine, H. and Foussard, J. N. (2000), Waste Manage. 20, 15–25.

    Article  CAS  Google Scholar 

  17. Schmidt, A. S., Puls, J., and Bjerre, A. B. (1996), in Biomass for Energy and the Environment, vol. 3, Chartier, P., Ferrero, G. L., Henius, U. M., Hultberg, S., Sachau, J., and Wiinblad, M., eds., Pergamon, Oxford, UK, pp. 1510–1515.

    Google Scholar 

  18. Schmidt, A. S. and Thomsen, A. B. (1998), Bioresour. Technol. 64, 139–151.

    Article  CAS  Google Scholar 

  19. Klinke, H. B., Ahring, B. K., Schmidt, A. S., and Thomsen, A. B. (2002), Bioresour. Technol. 82, 15–26.

    Article  PubMed  CAS  Google Scholar 

  20. Bjerre, A. B., Olesen, A. B., Fernqvist, T., Plöger, A., and Schmidt, A. S. (1996), Biotechnol. Bioeng. 49, 568–577.

    Article  CAS  Google Scholar 

  21. Kaylen, M., Van Dyne, D. L., Choi, Y.-S., and Blase, M. (2000), Bioresour. Technol. 72, 19–32.

    Article  CAS  Google Scholar 

  22. Stenberg, K., Tengborg, C., Galbe, M., and Zacchi, G. (1998), J. Chem. Technol. Biotechnol. 71, 299–308.

    Article  CAS  Google Scholar 

  23. IUPAC. (1987), Pure Appl. Chem. 59, 257–268.

    Google Scholar 

  24. Bailey, M. J. and Poutanen, K. (1992), J. Biotechnol. 23, 257–270.

    Article  CAS  Google Scholar 

  25. Stålbrand, H., Siika-aho, M., Tenkanen, M., and Viikari, L. (1993), J. Biotechnol. 29, 229–242.

    Article  Google Scholar 

  26. Poutanen, K. and Puls, J. (1988), Appl. Microbiol. Biotechnol. 28, 425–432.

    Article  CAS  Google Scholar 

  27. Poutanen, K., Rättö, M., Puls, J., and Viikari, L. (1987), J. Biotechnol. 6, 49–60.

    Article  CAS  Google Scholar 

  28. Rättö, M. and Poutanen, K. (1988), Biotechnol. Lett. 10, 661–664.

    Article  Google Scholar 

  29. Bailey, M. J. and Nevalainen, K. M. H. (1981), Enzyme Microb. Technol. 3, 153–157.

    Article  CAS  Google Scholar 

  30. Browning, B. L. (1967), Methods of Wood Chemistry, vols. I–II, Interscience Publications, New York.

    Google Scholar 

  31. Tenkanen, M. and Siika-aho, M. (2000), J. Biotechnol. 78, 149–161.

    Article  PubMed  CAS  Google Scholar 

  32. Bernfeld, P. (1955), in Methods in Enzymology, Colowick, S. P. and Kaplan, N. O., eds., Academic, New York, pp. 149–158.

    Chapter  Google Scholar 

  33. Tengborg, C., Stenberg, K., Galbe, M., Zacchi, G., Larsson, S., Palmqvist, E., and Hahn-Hägerdahl, B. (1998), Appl. Biochem. Biotechnol. 70–72, 3–15.

    Article  Google Scholar 

  34. Shevchenko, S. M., Chang, K., Robinson, J., and Saddler, J. N. (2000), Bioresour. Technol. 72, 207–211.

    Article  CAS  Google Scholar 

  35. Tengborg, C., Galbe, M., and Zacchi, G. (2001), Biotechnol. Prog. 17, 110–117.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. VTT Biotechnology, PO Box 1500, FIN-02044 VTT, Espoo, Finland

    Hetti Palonen, Maija Tenkanen & Liisa Viikari

  2. Plant Research Department, Risø National Laboratory, Roskilde, Denmark

    Anne Belinda Thomsen

  3. Novo Nordisk, Protein Chemistry, Hagedornsvej 1, DK-2820, Gentofte, Denmark

    Anette S. Schmidt

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  1. Hetti Palonen
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  2. Anne Belinda Thomsen
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  3. Maija Tenkanen
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  4. Anette S. Schmidt
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  5. Liisa Viikari
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Correspondence to Anne Belinda Thomsen.

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Palonen, H., Thomsen, A.B., Tenkanen, M. et al. Evaluation of wet oxidation pretreatment for enzymatic hydrolysis of softwood. Appl Biochem Biotechnol 117, 1–17 (2004). https://doi.org/10.1385/ABAB:117:1:01

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  • DOI: https://doi.org/10.1385/ABAB:117:1:01

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