Applied Biochemistry and Biotechnology

, Volume 117, Issue 1, pp 1–17 | Cite as

Evaluation of wet oxidation pretreatment for enzymatic hydrolysis of softwood

  • Hetti Palonen
  • Anne Belinda ThomsenEmail author
  • Maija Tenkanen
  • Anette S. Schmidt
  • Liisa Viikari
Original Articles


The wet oxidation pretreatment (water, oxygen, elevated temperature, and pressure) of softwood (Picea abies) was investigated for enhancing enzymatic hydrolysis. The pretreatment was preliminarily optimized. Six different combinations of reaction time, temperature, and pH were applied, and the compositions of solid and liquid fractions were analyzed. The solid fraction after wet oxidation contained 58–64% cellulose, 2–16% hemicellulose, and 24–30% lignin. The pretreatment series gave information about the roles of lignin and hemicellulose in the enzymatic hydrolysis. The temperature of the pretreatment, the residual hemicellulose content of the substrate, and the type of the commercial cellulase preparation used were the most important factors affecting the enzymatic hydrolysis. The highest sugar yield in a 72-h hydrolysis, 79% of theoretical, was obtained using a pretreatment of 200°C for 10 min at neutral pH.

Index Entries

Wet oxidation enzymatic hydrolysis lignocellulose cellulases 


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Copyright information

© Humana Press Inc. 2004

Authors and Affiliations

  • Hetti Palonen
    • 1
  • Anne Belinda Thomsen
    • 2
    Email author
  • Maija Tenkanen
    • 1
  • Anette S. Schmidt
    • 3
  • Liisa Viikari
    • 1
  1. 1.VTT BiotechnologyEspooFinland
  2. 2.Plant Research DepartmentRisø National LaboratoryRoskildeDenmark
  3. 3.Novo Nordisk, Protein ChemistryGentofteDenmark

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