Applied Biochemistry and Biotechnology

, Volume 114, Issue 1–3, pp 433–445 | Cite as

Enzymatic synthesis of monolaurin

  • Carla C. B. Pereira
  • Mônica A. P. da Silva
  • Marta A. P. Langone
Session 2—Introduction to Microbial Catalysis and Engineering


The aim of this study was to produce monolaurin utilizing a commercial immobilized lipase (Lipozyme IM-20; Novo Nordisk, Bagsvaerd, Denmark) through the direct esterification of lauric acid and glycerol in a solvent-free system. The influence of fatty acid/glycerol molar ratio, temperature, and Lipozyme (IM-20) concentration on the molar fraction of monolaurin were determined using an experimental design. The best conditions employed were 55°C, lauric acid/glycerol molar ratio of 1.0, and 3.0% (w/w) enzyme concentration. The final product, obtained after 6 h of reaction, was 45.5% monolaurin, 26.8% dilaurin, 3.1% trilaurin, and 24.6% lauric acid. The reusability of the enzyme was also studied.

Index Entries

Monolaurin Immobilized lipase esterification experimental design solvent-free medium 


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Copyright information

© Humana Press Inc. 2004

Authors and Affiliations

  • Carla C. B. Pereira
    • 1
  • Mônica A. P. da Silva
    • 1
  • Marta A. P. Langone
    • 2
  1. 1.Escola de QuímicaUniversidade Federal do Rio de Janeiro, Centro de TecnologiaRJBrazil
  2. 2.Instituto de QuímicaUniversidade do Estado do Rio de JaneiroRJBrazil

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