Applied Biochemistry and Biotechnology

, Volume 113, Issue 1–3, pp 299–305 | Cite as

Kinetics of asparaginase II fermentation in Saccharomyces cerevisiae ure2dal80 mutant

Effect of nitrogen nutrition and pH
  • Maria Antonieta Ferrara
  • Josiane M. V. Mattoso
  • Elba P. S. Bon
  • Nei PereiraJr.


Although the quality of nitrogen source affects fermentation product formation, it has been managed empirically, to a large extent, in industrial scale. Laboratory-scale experiments successfully use the high-cost proline as a nonrepressive source. We evaluated urea as a substitute for proline in Saccharomyces cerevisiae ure2dal80 fermentations for asparaginase II production as a model system for nitrogen-regulated external enzymes. Maximum asparaginase II levels of 265 IU/L were observed in early stationary-phase cells grown on either proline or urea, whereas in ammonium cells, the maximum enzyme level was 157 IU/L. In all cases, enzyme stability was higher in buffered cultures with an initial pH of 6.5.

Index Entries

Saccharomyces cerevisiae ure2dal80 mutants nitrogen sources asparaginase II fermentation kinetics 


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Copyright information

© Humana Press Inc. 2004

Authors and Affiliations

  • Maria Antonieta Ferrara
    • 1
  • Josiane M. V. Mattoso
    • 2
  • Elba P. S. Bon
    • 2
  • Nei PereiraJr.
    • 3
  1. 1.Far-Manguinhos, FIOCRUZRio de Janeiro, RJBrazil
  2. 2.Departamento de Bioquímica, Instituto de QuímicaUniversidade Federal do Rio de JaneiroBrazil
  3. 3.Escola de QuímicaUniversidade Federal do Rio de JaneiroRio de Janeiro, RJBrazil

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