Abstract
Novel microreactors with immobilized enzymes were fabricated using both silicon and polymer-based microfabrication techniques. The effectiveness of these reactors was examined along with their behavior over time. Urease enzyme was successfully incorporated into microchannels of a polymeric matrix of polydimethylsiloxane and through layer-bylayer self-assembly techniques onto silicon. The fabricated microchannels had cross-sectional dimensions ranging from tens to hundreds of micrometers in width and height. The experimental results for continuous-flow microreactors are reported for the conversion of urea to ammonia by urease enzyme. Urea conversions of > 90% were observed.
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Jones, F., Forrest, S., Palmer, J. et al. Immobilized enzyme studies in a microscale bioreactor. Appl Biochem Biotechnol 113, 261–272 (2004). https://doi.org/10.1385/ABAB:113:1-3:261
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DOI: https://doi.org/10.1385/ABAB:113:1-3:261