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Applied Biochemistry and Biotechnology

, Volume 113, Issue 1–3, pp 251–259 | Cite as

Characterization and performance of immobilized amylase and cellulase

  • Bradley A. SavilleEmail author
  • Mikhail Khavkine
  • Gayathri Seetharam
  • Behzad Marandi
  • Yong-Li Zuo
Article

Abstract

The performance of cellulase and amylase immobilized on siliceous supports was investigated. Enzyme uptake onto the support depended on the enzyme source and immobilization conditions. For amylase, the uptake ranged between 20 and 60%, and for cellulase, 7–10%. Immobilized amylase performance was assessed by batch kinetics in 100–300 g/L of corn flour at 65°C. Depending on the substrate and enzyme loading, between 40 and 60% starch conversion was obtained. Immobilized amylase was more stable than soluble amylase. Enzyme samples were preincubated in a water bath at various temperatures, then tested for activity. At 105°C, soluble amylase lost ∼55% of its activity, compared with ∼30% loss for immobilized amylase. The performance of immobilized cellulase was evaluated from batch kinetics in 10 g/L of substrate (shredded wastepaper) at 55°C. Significant hydrolysis of the wastepaper was also observed, indicating that immobilization does not preclude access to and hydrolysis of insoluble cellulose.

Index Entries

Amylase cellulase immobilization inactivation wastepaper 

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Copyright information

© Humana Press Inc. 2004

Authors and Affiliations

  • Bradley A. Saville
    • 1
    Email author
  • Mikhail Khavkine
    • 1
  • Gayathri Seetharam
    • 1
  • Behzad Marandi
    • 1
  • Yong-Li Zuo
    • 1
  1. 1.Department of Chemical Engineering and Applied ChemistryUniversity of TorontoTorontoCanada

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