Effect of temperature, moisture, and carbon supplementation on lipase production by solid-state fermentation of soy cake by Penicillium simplicissimum
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The production of lipases by Penicillium simplicissimum using solid-state fermentation and soy cake as substrate was investigated. The effects of temperature, cake moisture, and carbon supplementation on lipase production were studied using a two-level experimental plan. Moisture, pH, and lipase activity were followed during fermentation. Statistical analysis of the results was performed to evaluate the effect of the studied variables on the maximum lipase activity. Incubation temperature was the variable that most affected enzyme activity, showing a negative effect. Moisture and carbon supplementation presented a positive effect on activity. It was possible to obtain lipase activity as high as 21 U/g of dry cake in the studied range of process variables.
Index EntriesSolid-state fermentation lipase Penicillium soy cake olive oil moisture
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- 4.Del Bianchi, V. L., Moraes, I. O. and Capalbo, D. M. F. (2001), in Biotecnologia Industrial: Engenharia Bioquímica, vol. 3, Schmidell, W., Lima, U.A., Aquarone, E., and Borzani, W., eds., Edgard Blücher, São Paulo, Brazil.Google Scholar
- 5.Freire, D. M. G. (1996), PhD thesis, PEQ/COPPE/UFRJ, Rio de Janeiro, Brazil.Google Scholar
- 6.Freire, D. M. G., Gomes, P. M., Bon, E. P. S., and Sant’Anna, G. L., Jr. (1997), Revista Microbiologia 28(1), 6–12.Google Scholar
- 7.Leal, M. C. M. R. (2000), MS thesis, PEQ/COPPE/UFRJ, Rio de Janeiro, Brazil.Google Scholar
- 8.Pregnolatto, W., Pregnolatto, N. P. (coord.), and Rebocho, D. D. E. (ed.) (1985), Normas analíticas do Instituto Adolfo Lutz: vol. 1. Métodos químicos e físicios para análise de alimentos, 3rd Ed., Instituto Adolfo Lutz, São Paulo, Brazil.Google Scholar
- 9.MCT, Ministério da Ciênciae Tecnologia (Ministry of Science and Technology) (1985), Technical Report on Vegetable Oils, MCT.Google Scholar