Development and application of an integrated system for monitoring ethanol content of fuels
- 43 Downloads
An automated flow injection analysis (FIA) system for quantifying ethanol was developed using alcohol oxidase, horseradish peroxidase, 4-aminophenazone, and phenol. A colorimetric detection method was developed using two different methods of analysis, with free and immobilized enzymes. The system with free enzymes permitted analysis of standard ethanol solution in a range of 0.05–1.0 g of ethanol/L without external dilution, a sampling frequency of 15 analyses/h, and relative SD of 3.5%. A new system was designed consisting of a microreactor with a 0.91-mL internal volume filled with alcohol oxidase immobilized on glass beads and an addition of free peroxidase, adapted in an FIA line, for continued reuse. This integrated biosensor-FIA system is being used for quality control of biofuels, gasohol, and hydrated ethanol. The FIA system integrated with the microreactor showed a calibration curve in the range of 0.05–1.5 g of ethanol/L, and good results were obtained compared with the ethanol content measured by high-performance liquid chromatography and gas chromatography standard methods.
Index EntriesBiosensors gasohol immobilized enzymes alcohol oxidase horseradish peroxidase
Unable to display preview. Download preview PDF.
- 1.Nogueira, L. A. H. (2002), The Evolution of Fuel Quality in Brazil, 17 th World Petroleum Congress, (Website: http://www.ANP.gov.br), retrieved November 09, 2002.Google Scholar
- 2.Szwarc, A. (2002), Ethanol Usage in Automotive Fuels, World Fuels Conference—Latin America & the Caribbean, (Website: http://www.chemweek.com/worldfuels/rio/pdf/1408_03.pdf), retrieved on October 11, 2002.Google Scholar
- 12.ABNT. (1997), Brazilian Association of Technical Standards, NBR 13992.Google Scholar