Skip to main content
SpringerLink
Log in

Fermentation process optimization of recombinant Saccharomyces cerevisiae for the production of human interferon-α2a

  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

The effects of different culture conditions on the expression level of human interferon-α2a (IFN-α2a) by using recombinant yeast were investigated in a 2.6-Ljar fermentor. Appropriate supplement of glucose and the maintenance of residual glucose at a low level resulted in the reduction of ethanol formation and enhancement of the bioactivity of IFN-α2a to 4.9×106 from 3.1×106 IU/mL. When adenine was added evenlly for 10–20 h of fermentation into the basal culture medium at a speed of 2 μg/mL of medium/h, OD600 was greatly increased to 24, and the protein increased to 276 mg/L. The content of ethanol generated was also reduced tremendously during the process and as a result, 1.3×107 IU/mL of biologic activity was achieved. In the expression phase, pH had an important impact on expression level, which should be controlled at 5.5

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Wu, W. T., Ding, X. S., and Liu, J. J. (1996), in Genetic Engineering for Medicine, Wu, W. T., ed., People's Health Press, Beijing, pp. 154–161.

    Google Scholar 

  2. Kingsman, S. M. and Kingsman, A. J. (1983), Symp. Soc. Gen. Microbiol 35, 211–254.

    Google Scholar 

  3. Slocumbe, P., Easton, A., Boseley, P., and Burke, D. C. (1982), Proc. Natl. Acad. Sci. USA 79, 5455–5459.

    Article  Google Scholar 

  4. Tuite, M. F., Dobson, M. J., Roberts, N. A., King, R. M., Burke, D. C., Kingsman, S. M., and Kingsman, A. J. (1982), EMBO J. 1, 603–608.

    CAS  Google Scholar 

  5. Li, Y. (1987), Chin. J. Biotechnol. 3(2), 81.

    CAS  Google Scholar 

  6. Huo K.-K., Yu, L.-L., and Chen, X.-J. (1992), Sci. China, Ser. B 9:922–928.

    Google Scholar 

  7. Teo, W. K. and Yap, M. G. S. (1994), in Better Living Through Innovative Biochemical Engineering, Continental Press Pte Ltd., Singapore, pp. 357–362.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 200237, Shanghai, P.R. China

    Ju Chu, Siliang Zhang & Yingping Zhuang

Authors
  1. Ju Chu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Siliang Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yingping Zhuang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ju Chu.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chu, J., Zhang, S. & Zhuang, Y. Fermentation process optimization of recombinant Saccharomyces cerevisiae for the production of human interferon-α2a. Appl Biochem Biotechnol 111, 129–137 (2003). https://doi.org/10.1385/ABAB:111:3:129

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1385/ABAB:111:3:129

Index Entries

Search

Navigation