Applied Biochemistry and Biotechnology

, Volume 111, Issue 2, pp 81–91 | Cite as

Degradation of ferric EDTA by Burkhol cepacia

  • Hung-Yuan Fang
  • Shin-Chin Chen
  • Szu-Lin Chen


EDTA, the target compound of this study from the effluent of secondary biotreatment units, can be biodegraded by special microorganisms. So far, there are three species of microorganisms—Agrobacterium, Gram-negative BNCI, and DSM9103—that can degrade EDTA and are published in the literature. We have successfully isolated a bacterial strain that can degrade EDTA. It was identified as Burkhol cepacia, an aerobic species, elliptically shaped with a length of 5–15 μm. The growth medium contains 1000 mg/L of ferric-EDTA as carbon source, 750 mg/L of (NH4)2SO4+(NH2)2CO as nitrogen source, and 1000 mg/L of KH2PO4 as phosphorus source, and mineral factors such as Fe and Mg. Incubated at pH, 7.0, 30°C, and 150 rpm on a shaker for 15 d, the average specific growth rate of this microbe is 0.135 d−1, which shows that the respective degradation efficiency of Fe-EDTA and Cu-EDTA is 90 and 75% individually.

Index Entries

EDTA biodegradation Burkhol cepacia 


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  1. 1.
    Witschel, M., Nagel, S., and Egli, T. (1997), J. Bacteriol. 179(22), 6937–6943.Google Scholar
  2. 2.
    Kari, F. G. and Giger, W. (1996), Water Res. 30(1), 122–134.CrossRefGoogle Scholar
  3. 3.
    Payne, J. W., Bolton, H., Jr., Campbell, C., and Xum, L. (1998), J. Bacteriol. 180(15), 3823–3827.Google Scholar
  4. 4.
    Dietz, F. (1987), Wasser-GWF. 128, 286–288.Google Scholar
  5. 5.
    Gardiner, J. (1976), Water Res. 10, 507–514.CrossRefGoogle Scholar
  6. 6.
    Van Dijk-Looijaard, A. M., De Groot, A. C., Janssen, P. J. C. M., and Wondergem, E. A. (1990), RIVM report no 718629006, Rijksinstitut voor Voksgesondheid en Miliue, Bilthoven, The Netherlands.Google Scholar
  7. 7.
    Van Dijk-Looijaard, A. M., De Groot, A. C., Janssed, P. J. C. M., and Wondergem, E. A. (1990), H2O 33, 682–687.Google Scholar
  8. 8.
    Van Ginkel, C. G., Vandenbroucke, K. L., and Stroo, C. A. (1997), Bioresour. Technol. 59, 151–155.CrossRefGoogle Scholar
  9. 9.
    Egli, T. (1988), Microb. Sci. 5, 36–41.Google Scholar
  10. 10.
    Egli, T., Weilenman, H. U., El-Banna, T., and Auling, G. (1988), Syst. Appl. Microbiol. 10, 297–305.Google Scholar
  11. 11.
    Van Ginkel, C. G., Kester, H., Stroo, C. A., and van Haperen, A. M. (1999), Water Sci. Technol. 40(11–12), 259–265.Google Scholar
  12. 12.
    Thomas Russell, A. P. et al. (1998), Appl. Environ. Microbiol. 4, 1319–1322.Google Scholar
  13. 13.
    Bolton, H. Jr., Li, S. W., Workman, D. J., and Girvin, D. C. (1993), J. Environ. Quality 22, 125–132.CrossRefGoogle Scholar
  14. 14.
    Tiedje, J. M. (1975), Appl. Microbiol. 30, 327–329.Google Scholar
  15. 15.
    Palumbo, A. V., Lee, S. Y., and Boerman, P. (1994), Appl. Biochem. Biotechnol. 45, 811–822.Google Scholar
  16. 16.
    Lauff, J. J., Steele, D. B., Coogan, L. A., and Breitfeller, J. M. (1990), Appl. Environ. Microbiol. 56(11), 3346–3353.Google Scholar
  17. 17.
    Witschel, M., Egli, T., Zehnder, A. J. B., Wehrli, E., and Spycher, M. (1999), Microbiology 145, 973–983.CrossRefGoogle Scholar
  18. 18.
    Virtapohja, J. and Alen, R. (1999), Chemosphere 38(1), 143–154.CrossRefGoogle Scholar
  19. 19.
    APHA-AWWA-WPCF. (1992), Standard Methods for the Examination of Water and Wastewater, 18th ed. Washington DC, American Public Health Association, American Water Works Association and Water Pollution Control Federation.Google Scholar
  20. 20.
    Belly, R. T., Lauff, J. J., and Goodhue, C. T. (1975), Appl. Microbiol. 29, 787–794.Google Scholar
  21. 21.
    Lauff, J. J., Steele, D. B., Coogan, L. A., and Breitfeller, J. M. (1990), Appl. Environ. Microbiol. 56, 3346–3353.Google Scholar

Copyright information

© Humana Press Inc 2003

Authors and Affiliations

  1. 1.Graduate of Engineering Science and Technology School (Doctoral Program)National YunLin University of Science and TechnologyYunlinTaiwan
  2. 2.Department of Safety, Health, and Environmental EngineeringNational YunLin University of Science and TechnologyYunlinTaiwan

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