Applied Biochemistry and Biotechnology

, Volume 110, Issue 2, pp 91–100 | Cite as

Characterization of polysaccharide production of Haemophilus influenzae type b and its relationship to bacterial cell growth

  • Mickie Takagi
  • Joaquin Cabrera-Crespo
  • Júlia Baruque-Ramos
  • Teresa Cristina Zangirolami
  • Isaias Raw
  • Martha Massako Tanizaki


Haemophilus influenzae type b (Hib) causes invasive infections in infants and young children. Vaccines consisting of Hib capsular polysaccharide (polymer of ribosylribitol phosphate [PRP]) conjugated to a protein are effective in the prevention of such infections. The production of capsular polysaccharide type b was studied in three cultivation conditions: single, glucose pulse, and repeated batch. Specific polysaccharide production (Y p/x ) was calculated for all experiments, showing the following values: 67 (single-batch cultivation), 71 (glucose pulse), 75 (repeated-batch cultivation, first batch), and 87 mg of PRP/g of dry cell weight (DCW) (repeated-batch cultivation, second batch). Biomass concentration reached ∼1.8 g of DCW/L, while polysaccharide concentration was about ∼132 mg/L in the three fermentation runs. Polysaccharide synthesis is associated with cell growth in all studied conditions as established by Kono's analysis and Luedeking-Piret's model.

Index Entries

Haemophilus influenzae capsular polysaccharide polymer of ribosylribitol phosphate batch cultivation 


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Copyright information

© Humana Press Inc. 2003

Authors and Affiliations

  • Mickie Takagi
    • 1
  • Joaquin Cabrera-Crespo
    • 1
  • Júlia Baruque-Ramos
    • 1
  • Teresa Cristina Zangirolami
    • 2
  • Isaias Raw
    • 1
  • Martha Massako Tanizaki
    • 1
  1. 1.Laboratório de Fermentação Instituto ButantanCentro de BiotecnologiaSão PauloBrazil
  2. 2.Laboratório de Fermentação Departmento de Engenharia QuímicaUniversidade Federal de São CarlosSão CarlosBrazil

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