l-Asparaginase release from Escherichia coli cells with aqueous two-phase micellar systems
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A method was proposed to release and separate l-asparaginase (EC 22.214.171.124) from Escherichia coli ATCC 11303 cells with aqueous two-phase micellar systems. The systems were composed of K2HPO4 and Triton X-100. The method combines enzyme release with enzyme purification. The influence of Triton X-100 concentration, K2HPO4 concentration, and pH on the release and partition of l-asparaginase was investigated. Experimental results showed that E. coli cells treated with 9.4% (w/v) K2HPO4 and 15% (w/v) Triton X-100 at 25°C for 15–20 h released nearly 80% of the enzyme. Most of the released enzyme was partitioned to the bottom phase (phosphate-rich phase). The effects of Triton X-100 concentration, K2HPO4 concentration, and pH on cloud point were also studied. Electron micrography indicated that the chemical treatment altered the inner structure of E. coli cells significantly.
Index Entriesl-Asparaginase enzyme release Escherichia coli aqueous two-phase micellar system dipotassium hydrogen phosphate Triton X-100
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