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CelF of Orpinomyces PC-2 has an intron and encodes a cellulase (CelF) containing a carbohydrate-binding module

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Abstract

A cDNA, designated celF, encoding a cellulase (CelF) was isolated from the anaerobic fungus Orpinomyces PC-2. The open reading frame contains regions coding for a signal peptide, a carbohydrate-binding module (CBM), a linker, and a catalytic domain. The catalytic domain was homologous to those of CelA and CelC of the same fungus and to that of the Neocallimastix patriciarum CELA, but CelF lacks a docking domain, characteristic for enzymes of cellulosomes. It was also homologous to the cellobiohydrolase IIs and endoglucanases of aerobic organisms. The gene has a 111-bp intron, located within the CBM-coding region. Some biochemical properties of the purified recombinant enzyme are described.

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Authors and Affiliations

  1. Center for Biological Resource Recovery, Department of Biochemistry and Molecular Biology, The University of Georgia, Life Science Building, 30602-7229, Athens, GA

    Huizhong Chen & Lars G. Ljungdahl

  2. Fermentation Biotechnology Research Unit, NCAUR, USDA, ARS, 1815 N. University Street, 61604, Peoria, IL

    Xin-Liang Li

  3. Diversa Corporation, 4955 Directors Place, 92121-1609, San Diego, CA

    David L. Blum

  4. Laboratorio de Enzimlogia, Departamento de Biologia Celular, Universidade de Brasilia, Asa Norte, 70910-900, Brasilia-DF, Brazil

    Eduardo A. Ximenes

Authors
  1. Huizhong Chen
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  2. Xin-Liang Li
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  3. David L. Blum
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  4. Eduardo A. Ximenes
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  5. Lars G. Ljungdahl
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Correspondence to Xin-Liang Li.

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Names are necessary to report factually on available data; however, the USDA neither guarantees nor warrants the standard of the product, and the use of the name by USDA implies no approval of the product to the exclusion of others that may also be suitable.

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Chen, H., Li, XL., Blum, D.L. et al. CelF of Orpinomyces PC-2 has an intron and encodes a cellulase (CelF) containing a carbohydrate-binding module. Appl Biochem Biotechnol 108, 775–785 (2003). https://doi.org/10.1385/ABAB:108:1-3:775

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  • DOI: https://doi.org/10.1385/ABAB:108:1-3:775

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