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Protease production by Streptomyces sp. isolated from brazilian cerrado soil

Optimization of culture medium employing statistical experimental design

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Abstract

Streptomyces are important microorganisms because of their capacity to produce numerous bioactive molecules. In the present work protease production, by Streptomyces sp. 594 isolated from a Brazilian Cerrado soil, was maximized by optimizing a low-cost culture medium composition (casitone and sugarcane molasses) using statistical experimental design. The final protease activity (56 U/mL) was 2.8-fold and 58-fold higher than that obtained in the beginning of this study, and in a previous work, using an actinomycete selection medium, respectively. Protease production, not growth associated, appeared to be modulated by an inducer system, whereby the C/N ratio seemed to play a significant role.

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Authors and Affiliations

  1. Institute of Microbiology Prof. Paulo de Góes, USA

    Luciana A. I. de Azeredo & Rosalie R. R. Coelho

  2. Chemical Engineering Program, COPPE, USA

    Leda R. Castilho

  3. School of Chemistry, USA

    Selma G. F. Leite

  4. DBq/Instituto de Química, Universidade Federal do Rio de Janeiro, Prédio do Centro de Tecnologia, Bloco A, Lab. 549-2, Ilha do Fundão, 21949-900, Rio de Janeiro, RJ, Brasil

    Denise M. G. Freire

Authors
  1. Luciana A. I. de Azeredo
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  2. Leda R. Castilho
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  3. Selma G. F. Leite
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  4. Rosalie R. R. Coelho
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  5. Denise M. G. Freire
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Correspondence to Denise M. G. Freire.

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de Azeredo, L.A.I., Castilho, L.R., Leite, S.G.F. et al. Protease production by Streptomyces sp. isolated from brazilian cerrado soil. Appl Biochem Biotechnol 108, 749–755 (2003). https://doi.org/10.1385/ABAB:108:1-3:749

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  • DOI: https://doi.org/10.1385/ABAB:108:1-3:749

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