Abstract
Xylanase production of Trichoderma reesei Rut C-30 was examined at different initial pH values (4.8, 5.9, and 7.0) on rice straw in shake flasks, and in a fermentor, for the best pH condition. Enzyme performance was tested on ammonia-treated dwarf elephant grass. The maximum xylanase activities, 92 and 122 IU/mL, were obtained at pH 4.8 in the shake flasks and fermentor, respectively, in which good growth of the fungus was observed during the first 24 h and consumption of proteins dissolved from the rice straw caused the pH to rise later to values between 6.4 and 6.7 (optimal for xylanase production). The xylanases from T. reesei were as effective as Multifect XL, a commercial enzyme preparation, in hydrolyzing ammonia-treated elephant grass.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Kuhad, R. and Singh, A. (1993), Crit. Rev. Biotechnol. 13, 151–172.
Ryu, D. and Mandels, M. (1980), Enzyme Microb. Technol. 2, 91–102.
Wong, K. and Saddler, J. (1992), Crit. Rev. Biotechnol. 12, 413–435.
Gibbs, P., Serviour, R., and Schmid, F. (2000), Crit. Rev. Biotechnol. 20, 17–48.
Domingues, F., Quiroz, J., Cabral, J., and Fonseca, L. (2000), Enzyme. Microb. Technol. 26, 394–401.
Royer, J. and Nakas, J. (1989), Enzyme Microb. Technol. 11, 405–410.
Bailey, M., Buchert, J., and Viikari, l. (1993), Appl. Microbiol. Biotechnol. 40, 224–229.
Ministerio de Producción y Comercio. (2001), Estadísticas, Caracas, Venezuela.
Mandels, M. and Weber, J., (1969), Adv. Chem. Ser. 95, 391–414.
Ghosh, V. (1987), Pure Appl. Chem. 59, 257–268.
Bailey, M., Biely, Peters, and Poutanen, K. (1992), J. Biotechnol. 23, 257–270.
Lowry, O., Rosebrough, N., Farr, A., and Randall, R. (1965), Anal. Chem. 16, 190–210.
Szakacs, G. and Tengerdy, R. (1997), World J. Microbiol. Biotechnol. 13, 487–490.
Goering, H. and Van Soest, P. (1970), Agricultural Handbook, vol. 379, ARS-USDA, Washington, DC.
Ferrer, A., Byers, F., Sulbarán-de-Ferrer, B., Dale, B., and Aiello, C. (2000), Appl. Biochem. Biotechnol. 84–86, 163–179.
Miller, G. (1959), Anal. Chem. 31, 426–428.
Bracho, R., Colina, A., Sulbarán de Ferrer B., Ferrer, A., Parra, P., Peters, J., and Rumbos, C. (2001), in Memorias del V Congreso Venezolano de Química, Sociedad Venezulan de Química, Maracaibo, Venezuela, pp. 637–640.
Aiello, C., Ferrer, A., and Ledesma, A. (1996), Bioresourc. Technol. 57, 13–18.
Dekker, R. (1983), Biotechnol. Bioeng. 25, 1127–1146.
Bailey, M. and Poutanen, K. (1989), Appl. Microbiol. Biotechnol. 30, 5–10.
Gomes, I., Gomes, J., Steiner, W., and Esterbauer, H. (1992), Appl. Microbiol. Biotechnol. 36, 701–707.
Hayward, T., Hamilton, J., Templeton, D., Jennings, E., Ruth, M., Tholudur, A., McMillan, J., Tucker, M., and Mohagheghi, A. (1999), Appl. Biochem. Biotechnol. 84–86, 293–309.
Shin, C., Lee, J. P., Lee, J. S., and Park, S. (2000), Appl. Biochem. Biotechnol. 84–86, 237–245.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Colina, A., Sulbarán-de-Ferrer, B., Aiello, C. et al. Xylanase production by Trichoderma reesei rut C-30 on rice straw. Appl Biochem Biotechnol 108, 715–724 (2003). https://doi.org/10.1385/ABAB:108:1-3:715
Issue Date:
DOI: https://doi.org/10.1385/ABAB:108:1-3:715