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Lactic acid production through cell-recycle repeated-batch bioreactor

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Abstract

The effect of various nitrogen sources on cell growth and lactic acid production was investigated. The most effective nitrogen source was yeast extract; more yeast extract gave higher cell growth and lactic acid productivity. Yeast extract dosage and cell growth were proportional up to a yeast extract concentration of 30 g/L, and lactic acid productivity was linearly correlated up to a yeast extract dosage of 25 g/L. However, increasing the yeast extract content raises the total production cost of lactic acid. Therefore, we attempted to find the optimum yeast extract dosage for a repeated-batch operation with cell recycling. The results show that when using Enterococcus faecalis RKY1 only 26% of the yeast extract dosage for a conventional batch fermentation was sufficient to produce the same amount of lactic acid, whereas the lactic acid concentration in the product stream (92–94 g/L) and lactic acid productivity (6.03–6.20 g/[L·h]) were similar to those of a batch operation. Furthermore, long-term stability was established.

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Authors and Affiliations

  1. Faculty of Applied Chemical Engineering, Institute of Bioindustrial Technology, Chonnam National University, 500-757, Gwangju, Korea

    Hurok Oh, Young-Jung Wee, Jong-Sun Yun & Hwa-Won Ryu

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  1. Hurok Oh
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  2. Young-Jung Wee
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  3. Jong-Sun Yun
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  4. Hwa-Won Ryu
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Correspondence to Hwa-Won Ryu.

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Oh, H., Wee, YJ., Yun, JS. et al. Lactic acid production through cell-recycle repeated-batch bioreactor. Appl Biochem Biotechnol 107, 603–613 (2003). https://doi.org/10.1385/ABAB:107:1-3:603

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  • DOI: https://doi.org/10.1385/ABAB:107:1-3:603

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