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d-xylose transport by Candida succiphila and Kluyveromyces marxianus

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Abstract

The kinetics and regulation of d-xylose uptake were investigated in the efficient pentose fermentor Candida succiphila, and in Kluyveromyces marxianus, which assimilate but do not ferment pentose sugars. Active high-affinity (K m ∼ 3.8 mM; V max ∼ 15 nmol/[mg·min]) and putative facilitated diffusion low-affinity (K m ∼ 140 mM; V max ∼ 130 nmol/[mg·min]) transport activities were found in C. succiphila grown, respectively, on xylose or glucose. K. marxianus showed facilitated diffusion low-affinity (K m ∼ 103 mM; V max ∼ 190 nmol/[mg·min]) transport activity when grown on xylose under microaerobic conditions, and both a low-affinity and an active high-affinity (K m ∼ 0.2 mM; V max ∼ 10 nmol/[mg·min]) transport activity when grown on xylose under fully aerobic conditions.

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Correspondence to Boris U. Stambuk.

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Stambuk, B.U., Franden, M.A., Singh, A. et al. d-xylose transport by Candida succiphila and Kluyveromyces marxianus . Appl Biochem Biotechnol 106, 255–263 (2003). https://doi.org/10.1385/ABAB:106:1-3:255

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