Applied Biochemistry and Biotechnology

, Volume 105, Issue 1–3, pp 155–164 | Cite as

Enzymatic hydrolysis of ammonia-treated rice straw

  • Betzabé Sulbarán-de-Ferrer
  • Marielena Aristiguieta
  • Bruce E. Dale
  • Alexis Ferrer
  • Graciela Ojeda-de-Rodriguez


Rice straw pretreated with liquid anhydrous ammonia was hydrolyzed with cellulase, cellobiase, and hemicellulase. Ammonia-processing conditions were 1.5 g of NH3/g of dry matter, 85°C, and several sample moisture contents. There were four ammonia addition time (min)-processing time (min) combinations. Sugars produced were analyzed as reducing sugars (dinitrosalicylic acid method) and by high-performance liquid chromatography. Monomeric sugars increased from 11% in the nontreated rice straw to 61% of theoretical in treated rice straw (79.2% conversion as reducing sugars). Production of monosaccharides was greater at higher moisture content and was processing time dependent. Glucose was the monosaccharide produced in greater amounts, 56.0%, followed by xylose, arabinose, and fructose, with 35.8, 6.6, and 1.4%, respectively.

Index Entries

Sugars rice straw ammonia treatment 


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Copyright information

© Humana Press Inc. 2003

Authors and Affiliations

  • Betzabé Sulbarán-de-Ferrer
    • 1
  • Marielena Aristiguieta
    • 1
  • Bruce E. Dale
    • 2
  • Alexis Ferrer
    • 1
  • Graciela Ojeda-de-Rodriguez
    • 1
  1. 1.Lab. de Alimentos, Dept. de Química, Facultad de CienciasUniversidad del ZuliaMaracaiboVenezuela
  2. 2.Department of Chemical EngineeringMichigan State UniversityEast Lansing

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