Applied Biochemistry and Biotechnology

, Volume 102, Issue 1–6, pp 407–414 | Cite as

Culture conditions dictate protease and tannase production in submerged and solid-state cultures of Aspergillus niger Aa-20

  • Cristóbal Noé Aguilar
  • Ernesto Favela-Torres
  • Gustavo Vinegra-González
  • Christopher Augur
Article

Abstract

Undesirable protease production by Aspergillus niger Aa-20 in submerged culture and solid-state culture was evaluated using different concentrations of tannic acid as sole carbon source in a model system designed for tannase production. Protease production was found to be dependent on the culture system used (submerged culture or solid-state culture) and on the initial tannic acid concentration. Expression of protease activity in submerged culture was higher (up to 10 times) than activity obtained in solid-state culture, using identical culture medium composition. In submerged culture, the lowest final protease activity (0.13 IU) was obtained with the highest tannic acid concentration, while in solid-state culture protease activity was not affected by changes in initial substrate concentration. Absence of detectable proteolytic activity in solid-state culture is related to high production of tannase enzyme. Hence, the use of solid-state culture for fungal enzyme production may allow for higher and more stable enzyme titers present in culture extracts.

Index Entries

Protease solid-state fermentation tannase tannin acyl hydrolase liquid fermentation submerged fermentation Aspergillus niger tannic acid 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Romero-Gómez, S. J., Augur, C., and Viniegra-González, G. (2000), Biotechnol. Lett. 22(15), 1255–1258.CrossRefGoogle Scholar
  2. 2.
    Ramesh, M. V. and Lonsane, B. K. (1991), Appl. Microbiol. Biotechnol. 35, 591–593.CrossRefGoogle Scholar
  3. 3.
    Solís-Pereira, S., Favela-Torres, E., Viniegra-González, G., and Gutiérrez-Rojas, M. (1993), Appl. Microbiol. Biotechnol. 39, 36–41.Google Scholar
  4. 4.
    Maldonado, M. C. and Strasser de Saad, A. M. (1998), J. Ind. Microbiol. Biotechnol. 20, 34–38.PubMedCrossRefGoogle Scholar
  5. 5.
    Lekha, P. K. and Lonsane, B. K. (1994), Proc. Biochem. 29, 497–503.CrossRefGoogle Scholar
  6. 6.
    George, S., Raju, V., Subramanian, V., and Jarayaman, K. (1997), Bioprocess. Eng. 16, 381, 382.CrossRefGoogle Scholar
  7. 7.
    Alazard, D. and Raimbault, M. (1981), Eur. J. Appl. Microbiol. Biotechnol. 12, 113–117.CrossRefGoogle Scholar
  8. 8.
    Grajek, W. and Gervais, P. (1987), Appl. Microbiol. Biotechnol. 26, 537–541.CrossRefGoogle Scholar
  9. 9.
    Oriol, E., Raimbault, M., Roussos, S., and Viniegra-González, G. (1988), Appl. Microbiol. Biotechnol. 27, 498–503.Google Scholar
  10. 10.
    Aguilar, C. N. (1999), Arch. Latinoam. Microbiol. 41, 10–21.Google Scholar
  11. 11.
    Aguilar, C. N., Augur, C., Favela-Torres, E., and Viniegra-González, G. (2001), Proc. Biochem. 36, 571–578.CrossRefGoogle Scholar
  12. 12.
    Aguilar, C. N., Augur, C., Favela-Torres, E., and Viniegra-González, G. (2001), J. Ind. Microbiol. Biotechnol. 26, 296–302.PubMedCrossRefGoogle Scholar
  13. 13.
    Klapper, B. F., Jameson, D. M., and Mayer, R. M. (1973), Biochim. Biophys. Acta 304, 513–519.PubMedGoogle Scholar
  14. 14.
    Nakadai, T. and Nasuno, S. (1988), J. Ferment. Technol. 66, 525–533.CrossRefGoogle Scholar
  15. 15.
    Fukushima, Y., Itoh, H., Fukase, T., and Motai, H. (1989), Appl. Microbiol. Biotechnol. 30, 604–608.CrossRefGoogle Scholar
  16. 16.
    Aikat, K. and Bhattacharyya, B. C. (2000), Acta Biotechnologica 20(2), 149–159.CrossRefGoogle Scholar
  17. 17.
    Thakur, M. S., Karanth, N. G., and Nand, K. (1990), Appl. Microbiol. Biotechnol. 32, 409–413.CrossRefGoogle Scholar
  18. 18.
    Battaglino, R. A., Huergo, M., Pilosuf, A. M. R., and Bartholomai, G. B. (1991), Appl. Microbiol. Biotechnol. 35, 292–296.CrossRefGoogle Scholar
  19. 19.
    Malathi, S. and Chakraverty, R. (1991), Appl. Environ. Microbiol. 57, 712–716.PubMedGoogle Scholar
  20. 20.
    Ikasari, L. and Mitchell, D. A. (1994), World J. Microbiol. Biotechnol. 10, 320–324.CrossRefGoogle Scholar
  21. 21.
    Tunga, R., Banerjee, R., and Bhattacharyya, B. C. (1998), Bioprocess. Eng. 19, 187–190.CrossRefGoogle Scholar
  22. 22.
    Mitra, P., Chakraverty, R., and Chandra, A. L. (1996), J. Sci. Ind. Res. 55, 439–442.Google Scholar
  23. 23.
    Godfrey, T. and Reichelt, J. R. (1983), in Industrial Enzymology, Nature Press, New York, pp. 1–7.Google Scholar
  24. 24.
    Kim, H., Kim, K., Lee, J., Bae, K., Sung, C., and Oh, T. (1994), J. Microbiol. Biotechnol. 4, 113–118.Google Scholar
  25. 25.
    Ward, O. P. (1983), in Microbial Enzymes and Biotechnology, Fogarty, W., ed., Elsevier Applied Sciences, London, pp. 251–317.Google Scholar
  26. 26.
    Yang, S. S and Chiu, W. F. (1986), Chin. J. Microbiol. Immunol. 19, 276–288.Google Scholar
  27. 27.
    Yang, S. S. and Wang, J. Y. (1999), Bot. Bull. Acad. Sin. 40, 259–265.Google Scholar
  28. 28.
    Couri, S., da Costa-Terzi, S., Saavedra-Pinto, G. A., Pereira-Freitas, S., and Augusto da Costa, A. C. (2000), Proc. Biochem. 36, 255–261.CrossRefGoogle Scholar
  29. 29.
    Zhu, Y., Smith, J., Knol, W., and Bol, J. (1994), Biotechnol. Lett. 16, 643–648.CrossRefGoogle Scholar
  30. 30.
    Dosoretz, C., Chih-Chen, H., and Grethlein, H. (1990), Appl. Environ. Microbiol. 56, 395–400.PubMedGoogle Scholar
  31. 31.
    Beverini, M. and Metche, M. (1990), Sci. Aliments 10, 807–816.Google Scholar
  32. 32.
    Layman, P. (1990), Chem. Eng. News 68, 17, 18.Google Scholar

Copyright information

© Humana Press Inc. 2002

Authors and Affiliations

  • Cristóbal Noé Aguilar
    • 1
    • 2
  • Ernesto Favela-Torres
    • 2
  • Gustavo Vinegra-González
    • 2
  • Christopher Augur
    • 3
  1. 1.Food Research Department, Faculty of Chemical SciencesUniversidad Autónoma de Coahuila, Unidad Saltillo, Coah.México
  2. 2.Biotechnology DepartmentUniversidad Autónoma Metropolitana, Unidad IztapalapaMéxico, D.F.
  3. 3.Institut de Recherche pour le DéveloppementIRD-France CESB/ESILMarseille, Cedex 9France

Personalised recommendations