Applied Biochemistry and Biotechnology

, Volume 102, Issue 1–6, pp 407–414 | Cite as

Culture conditions dictate protease and tannase production in submerged and solid-state cultures of Aspergillus niger Aa-20

  • Cristóbal Noé Aguilar
  • Ernesto Favela-Torres
  • Gustavo Vinegra-González
  • Christopher Augur


Undesirable protease production by Aspergillus niger Aa-20 in submerged culture and solid-state culture was evaluated using different concentrations of tannic acid as sole carbon source in a model system designed for tannase production. Protease production was found to be dependent on the culture system used (submerged culture or solid-state culture) and on the initial tannic acid concentration. Expression of protease activity in submerged culture was higher (up to 10 times) than activity obtained in solid-state culture, using identical culture medium composition. In submerged culture, the lowest final protease activity (0.13 IU) was obtained with the highest tannic acid concentration, while in solid-state culture protease activity was not affected by changes in initial substrate concentration. Absence of detectable proteolytic activity in solid-state culture is related to high production of tannase enzyme. Hence, the use of solid-state culture for fungal enzyme production may allow for higher and more stable enzyme titers present in culture extracts.

Index Entries

Protease solid-state fermentation tannase tannin acyl hydrolase liquid fermentation submerged fermentation Aspergillus niger tannic acid 


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Copyright information

© Humana Press Inc. 2002

Authors and Affiliations

  • Cristóbal Noé Aguilar
    • 1
    • 2
  • Ernesto Favela-Torres
    • 2
  • Gustavo Vinegra-González
    • 2
  • Christopher Augur
    • 3
  1. 1.Food Research Department, Faculty of Chemical SciencesUniversidad Autónoma de Coahuila, Unidad Saltillo, Coah.México
  2. 2.Biotechnology DepartmentUniversidad Autónoma Metropolitana, Unidad IztapalapaMéxico, D.F.
  3. 3.Institut de Recherche pour le DéveloppementIRD-France CESB/ESILMarseille, Cedex 9France

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