Applied Biochemistry and Biotechnology

, Volume 102, Issue 1–6, pp 303–313 | Cite as

Immobilization of α-amylase from Bacillus circulans GRS 313 on coconut fiber

  • Gargi Dey
  • Varima Nagpal
  • Rintu Banerjee


A simple and inexpensive method for immobilizing α-amylase from Bacillus circulans GRS 313 on conconut fiber was developed. The immobilization conditions for highest efficiency were optimized with respect to immobilization pH of 5.5, 30°C, contact time of 4 h, and enzyme to support a ratio of 1:1 containing 0.12 mg/mL of protein. The catalytic properties of the immobilized enzyme were compared with that of the free enzyme. The activity of amylase adsorbed on coconut fiber was 38.7 U/g of fiber at its optimum pH of 5.7 and 48°C, compared with the maximum activity of 40.2 U/mL of free enzyme at the optimum pH of 4.9 and 48°C. The reutilization capacity of the immobilized enzyme was up to three cycles.

Index Entries

Coconut fiber adsorption response surface methodology amylase Bacillus circulans GRS 313 


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Copyright information

© Humana Press Inc. 2002

Authors and Affiliations

  1. 1.Microbial Biotechnology & Downstream Processing LaboratoryAgricultural & Food Engineering DepartmentIIT-KharagpurIndia

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