Abstract
Xylitol production by Debaryomyces hansenii NRRL Y-7426 was performed on synthetic medium varying the initial xylose concentration between 50 and 300 g/L. The experimental results of these tests were used to investigate the effect of substrate level on xylose consumption by this yeast. Satisfactory values of product yield on substrate (0.74–0.83 g/g) as well as volumetric productivity (0.481–0.694 g/L·h) were obtained over a wide range of xylose levels (90–200 g/L), while a worsening of kinetic parameters took place at higher concentration, likely due to a substrate inhibition phenomenon. The metabolic behavior of D. hansenii was studied, under these conditions, through a carbon material balance to estimate the fractions of xylose consumed by the cell for different activities (xylitol production, biomass growth, and respiration) during the lag, exponential, and stationary phases.
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Converti, A., Perego, P., Sordi, A. et al. Effect of starting xylose concentration on the microaerobic metabolism of Debaryomyces hansenii . Appl Biochem Biotechnol 101, 15–29 (2002). https://doi.org/10.1385/ABAB:101:1:15
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DOI: https://doi.org/10.1385/ABAB:101:1:15