Abstract
A high-performance and rapid chemiluminescence immunoassay for model food allergen lysozyme, one of the major allergenic components in egg white, using antibody-conjugated bacterial magnetic particles and a fully automated system was developed. This system contians a reaction station, tip rack, and an eight-tip pipettor that is alble to attach and detach a strong magnet to the tip surface. The immunoreaction time was shortened to 5 min, and the assay was completed within 20 min. The lower detection limit for lysozyme was 10 ng/mL. This system can be used to perform 24 samples in 60 min within 10% coefficient of variation.
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Sato, R., Takeyama, H., Tanaka, T. et al. Development of high-performance and rapid immunoassay for model food allergen lysozyme using antibody-conjugated bacterial magenetic particles and fully automated system. Appl Biochem Biotechnol 91, 109–116 (2001). https://doi.org/10.1385/ABAB:91-93:1-9:109
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DOI: https://doi.org/10.1385/ABAB:91-93:1-9:109