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Preparation of γ-aminobutyric acid using E. coli cells with high activity of glutamate decarboxylase

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Abstract

γ-Aminobutyric acid (GABA) is a major inhibitory neurotransmitter synthesized in the central nervous system from glutamate by glutamate decarboxylase (GAD). It has applications in the production of many drugs. The technology of GABA synthesis by treating L-glutamic acid with the cells of the gene-engineered GAD superproducer strain of Escherichia coli GAD K10 was developed. Cell growing in the presence of 0.02 mM pyridoxal phosphate (PLP) causes the 2- to 2.5-fold increase of total productivity of the cells. The best way to prepare the cells for the reaction was their thermal activation by pretreatment for 1 h at 53°C. The optimal conditions for this reaction were 37°C and pH 4.6. The rate of the enzymatic reaction is the function of acetate concentration with the maximum at 0.5 M acetate. The total amount of GABA synthesized using 1 g of wet cells reached 23–25 g. The final concentration of GABA in the reaction medium was 280–300 g/L. The yield of the product was about 99%.

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References

  1. Sytinsky, I. A. (1972), γ-Aminobutyric Acid and Activity of the Central Nervous System, Leningrad, Nauka.

    Google Scholar 

  2. Krnjevic, K. (1974), Physiol. Rev. 54, 418–540.

    CAS  Google Scholar 

  3. Roberts, E. (1974), Biochem. Pharm. 23, 2637–2649.

    Article  CAS  Google Scholar 

  4. Camien, M. N., Mc Clure, L. E., Lepp, A., and Dunn, M. S. (1953), Arch. Biochem. Biophys. 43, 378–380.

    Article  CAS  Google Scholar 

  5. Gubarev, E. M., and Galaev, Yu. V. (1960), Biokhimiya (Moscow) 25, 261–263.

    Google Scholar 

  6. Schulga, A. A., Kurbanov, F. T., Khristoforov, R. R., Darii, E. L., and Sukhareva, B. S. (1999), Mol. Biol. (Moscow) 33, 560–566.

    Google Scholar 

  7. Bradford, M. (1976), Anal. Biochem. 72, 248.

    Article  CAS  Google Scholar 

  8. Erdman, I. E., Nys, P. S., and Biryukov, V. V. (1986), Antibiotics Med. Biotechnol. 4, 249–254.

    Google Scholar 

  9. Sukhareva, B. S., and Braunstein, A. E. (1971), Mol. Biol. 5, 302–317.

    CAS  Google Scholar 

  10. Strausbauch, P. N. and Fisher, E. (1970), Biochemistry 9, 226–233.

    Article  CAS  Google Scholar 

  11. Shukuya, R. and Schwert, G. W. (1960), J. Biol. Chem. 235, 123–126.

    Google Scholar 

  12. Fonda, M. L. (1972), Arch. Biochem. Biophys. 153, 763–768.

    Article  CAS  Google Scholar 

  13. Lambrecht, R. H. D., Slegers, G., Mannens, G., and Claes A. (1987), Enzyme Microb. Technol. 9, 221–224.

    Article  CAS  Google Scholar 

  14. Khristoforov, R. R., Sukhareva, B. S., Dixon, H. B. F., Sparkes, M. J., Krasnov, V. P. and Bukrina, I. M. (1995), Biochem. Mol. Biol. Intern. 36, 77–85.

    CAS  Google Scholar 

  15. De Biase, D., Tramonti, A., John, A. R., and Bossa, F. (1996), prot. Expr. Purif. 8, 430–438.

    Article  Google Scholar 

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Correspondence to A. Yu. Plokhov.

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Plokhov, A.Y., Gusyatiner, M.M., Yampolskaya, T.A. et al. Preparation of γ-aminobutyric acid using E. coli cells with high activity of glutamate decarboxylase. Appl Biochem Biotechnol 88, 257–265 (2000). https://doi.org/10.1385/ABAB:88:1-3:257

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  • DOI: https://doi.org/10.1385/ABAB:88:1-3:257

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