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Use of corncob for endoxylanase production by thermophilic fungus Thermomyces lanuginosus IOC-4145

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Abstract

The production of cellulase-free end oxylanase by the thermophilic fungus Thermomyces lanuginosus was investigated insemisolid fermentation and liquid fermentation. Different process variables were investigated in semisolid fermentation, employing corncobas the carbon source. The best results were with the following conditions: grain size=4.5 mm, solid:liquid ratio=1:2, and inoculum size=20% (v/v). Corncob, xylan, and xylose were the best inducers for endoxylanase production. Additionally, organic nitrogen sources were necessary for the production of high endoxylanase activities. The crude enzyme had optimum activity at pH 6.0 and 75°C, displaying a high thermostability. The apparent K 25 and V max were 1.77 mg of xylan/mL and 21.5 U/mg of protein, respectively.

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Authors and Affiliations

  1. Escola de Química da Universidade Federal do Rio de Janeiro, PO Box 68542, CEP 21945-970, Rio de Janeiro, RJ, Brazil

    Mônica C. T. Damaso, Carolina M. M. C. Andrade & Nei Pereira Jr.

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  1. Mônica C. T. Damaso
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  2. Carolina M. M. C. Andrade
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  3. Nei Pereira Jr.
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Correspondence to Nei Pereira Jr..

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Damaso, M.C.T., Andrade, C.M.M.C. & Pereira, N. Use of corncob for endoxylanase production by thermophilic fungus Thermomyces lanuginosus IOC-4145. Appl Biochem Biotechnol 84, 821–834 (2000). https://doi.org/10.1385/ABAB:84-86:1-9:821

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  • DOI: https://doi.org/10.1385/ABAB:84-86:1-9:821

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