Skip to main content
SpringerLink
Log in

A high-copy-number plasmid capable of replication in thermophilic cyanobacteria

  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

A 2.5 kb high-copy-number plasmid, pM A4 in thermophilic cyanobacterium Synechococcus sp. M A4 was isolated and characterized to develop a genetic engineering system for thermophilic cyanobacteria. The copy number of pM A4 was determined to be by densitometry about 350/cell. The pM A4 may be a type of rolling-circle plasmid, because a possible rep gene encoding 34 k D-protein and a consensus sequence of a double-stranded origin nick site of rolling circle plasmids were found in the pM A4 sequence. The pM A4 was electro-introduced into another thermophile, Synechococcus sp. MA 19, which is the strongest poly-β-hydroxybutyrate (PHB) accumulator in photoau totrophic organisms. The pM A4 was incorporated and retained in MA 19. These results indicate that pM A4 could be developed as a useful vector for thermophilic cyanobacteria.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Borowitzka, M. A. and Borowitzka, L. J. (1988), Micro-algal Biotechnology, Cambridge University Press, New York.

    Google Scholar 

  2. Asada, Y. (1998), in Studies in Surface Science and Catalysis, vol. 114, Inui, T., Anpo, M., Izui, K., Yanagida, S., and Yamaguchi, T., eds., Elsevier, Amsterdam, pp. 321–326.

    Google Scholar 

  3. Tandeau de Marsac, N., de la Torre, F., and Szulmajster, J. (1987), J. Mol. Gen. Genet. 209, 396–398.

    Article  CAS  Google Scholar 

  4. Chungjatupornchai, W. (1990), Curr. Microbiol. 21, 283–288.

    Article  CAS  Google Scholar 

  5. Murphy, R. C. and Stevens Jr., S. E. (1992), Appl. Env. Microbiol. 58, 1650–1655.

    CAS  Google Scholar 

  6. Xudong, X., Rennqiu, K., and Yuxiang, H. (1993), FEMS Microbiol. Lett. 103, 247–250.

    Google Scholar 

  7. Kawata, Y., Yamano, N., Kojima, H., and Itoh, S. (1991), Biotechnol. Lett. 13, 851–856.

    Article  CAS  Google Scholar 

  8. Elanskaya, I. V. and Morzunova, I. B. (1989), Mol. Genet. Mikrobiol. Virusol. 9, 7–11.

    Google Scholar 

  9. Takeyama, H., Takeda, D., Yazawa, K., Yamada, A., and Matsunaga, T. (1997), Microbiology 143, 2725–2731.

    Article  CAS  Google Scholar 

  10. Sakai, M., Ogawa, T., Matsuoka, M., and Fukuda, H. (1997), J. Ferment. Bioeng. 84, 434–443.

    Article  CAS  Google Scholar 

  11. Suzuki, T., Miyake, M., Tokiwa, Y., Saegusa, H., Saito, T., and Asada, Y. (1996), Biotech. Lett. 18, 1047–1050.

    Article  CAS  Google Scholar 

  12. Hayashi, N. R., Peeraponpisal, Y., Nishihara, H., Ishii, M., Igarashi, Y., and Kodama, T. (1994), J. Ferment. Bioeng. 78, 179–181.

    Article  Google Scholar 

  13. Miyake, M., Erata, M., and Asada, Y. (1996), J. Ferment. Bioeng. 82, 516–518.

    Google Scholar 

  14. Brandl, H., Gross, R. A., Lenz, R. W., and Fuller, R. C. (1990), Adv. Biochem. Eng. Biotechnol. 41, 77–93.

    CAS  Google Scholar 

  15. Miyake, M. and Asada, Y. (1996), J. Marine Biotechnol. 4, 113–116.

    CAS  Google Scholar 

  16. Muhlenhoff, U. and Chauvat, F. (1996), J. Mol. Gen. Genet. 252, 93–100.

    Article  CAS  Google Scholar 

  17. Miyake, M., Kataoka, K., Shirai, M., and Asada, Y. (1997), J. Bacteriol. 179, 5009–5013.

    CAS  Google Scholar 

  18. Mac, Y. M. and Ho, K. K. (1991), Nucleic Acids Res. 20, 4101–4102.

    Google Scholar 

  19. Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989), Molecular Cloning: A Laboratory Manual, 2nd ed., Cold Spring Harbor Laboratory Press, Cold Springer Harbor, NY.

    Google Scholar 

  20. Allen, M. M. (1968), J. Physiol. 4, 1–4.

    CAS  Google Scholar 

  21. Yanisch-Perron, C., Vieira, J., and Messing, J. (1985), Gene 33, 103–119.

    Article  CAS  Google Scholar 

  22. Yamaguchi, Y. and Yamaguchi, M. (1984), Gene 29, 211–219.

    Article  CAS  Google Scholar 

  23. Lau, R. H. and Straus, N. A. (1985), FEMS Microbiol. Lett. 27, 253–256.

    Article  CAS  Google Scholar 

  24. Masepohl, B., Gorlitz, K., and Bohme, H. (1996), Biochim. Biophys. Acta 1307, 26–30.

    Google Scholar 

  25. Seery, L. T., Nolan, N. C., Sharp, P. M., and Devine, K. (1993), Plasmid 30, 185–196.

    Article  CAS  Google Scholar 

  26. Walton, D. K., Gendel, S. M., and Atherly, A. G. (1993), Nucleic Acids Res. 21, 746.

    Article  CAS  Google Scholar 

  27. Perkins, D. J. and Barnum, S. R. (1992), Plasmid 28, 170–176.

    Article  CAS  Google Scholar 

  28. Yang, X. and McFadden, B. (1993), J. Bacteriol. 175, 3981–3991.

    CAS  Google Scholar 

  29. Nedved, M. L., Gottlieb, P. A., and Moe, G. R. (1994), Nucleic Acids Res. 22, 5024–5030.

    Article  CAS  Google Scholar 

  30. Carlson, D. M. (1993), Crit. Rev. Oral Biol. Med. 4, 495–502.

    CAS  Google Scholar 

  31. Kauffman, D. L., Keller, P. J., Bennick, A., and Blum, M. (1993), Crit. Rev. Oral Biol. Med. 4, 287–292.

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. National Institute of Bioscience and Human-Technology, Agency of Industrial Science and Technology, Ministry of International Trade and Industry, Higashi 1-1, 305-8566, Tsukuba, Ibaraki, Japan

    Masato Miyake, Ryuichiro Kurane & Yasuo Asada

  2. Division of Biotechnology, Ibaraki University, Ibaraki, Japan

    Hiroshi Nagai & Makoto Shirai

Authors
  1. Masato Miyake
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hiroshi Nagai
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Makoto Shirai
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ryuichiro Kurane
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yasuo Asada
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Yasuo Asada.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Miyake, M., Nagai, H., Shirai, M. et al. A high-copy-number plasmid capable of replication in thermophilic cyanobacteria. Appl Biochem Biotechnol 77, 267–275 (1999). https://doi.org/10.1385/ABAB:77:1-3:267

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1385/ABAB:77:1-3:267

Index Entries

Search

Navigation