Skip to main content
SpringerLink
Log in

Evaluation of recombinant strains of Zymomonas mobilis for ethanol production from glucose/xylose media

  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

The fermentation characteristics of two recombinant strains of Zymomonas mobilis, viz. CP4 (pZB5) and ZM4 (pZB5), capable of converting both glucose and xylose to ethanol, have been characterized in batch and continuous culture studies. The strain ZM4 (pZB5) was found to be capable of converting a mixture of 65 g/L glucose and 65 g/L xylose to 62 g/L ethanol in 48h with a yield of 0.46 g/g. Higher sugar concentrations resulted in incompletexylose utilization (80h) presumably owing to ethanol inhibition of xylose assimilation or metabolism. The fermentation results with ZM4 (pZB5) show a significant improvement over results published previously for recombinant yeasts and other bacteria capable of glucose and xylose utilization.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Toon, S., Philippidis, G. P., Ho, N. W. Y., Chen, Z. D., Brainard, A., Lumpkin, R. E., and Riley, C. J. (1996), Appl. Biochem. Biotechnol. 63/65, 243–255.

    Google Scholar 

  2. Lindsay, S. E., Bothast, R. J., and Ingram, L. O. (1995), Appl. Microbiol. Biotechnol. 43, 70–75.

    Article  CAS  Google Scholar 

  3. Ohta, K., Beall, D. S., Meijia, J. P., Shannugan, T., and Ingram, L. O. (1991), Appl. Env. Microbiol., 57, 2810–2815.

    CAS  Google Scholar 

  4. Zhang, M., Eddy, C., Deanda, K., Finkelstein, M., and Picataggio, S. (1995), Science 267, 240–243.

    Article  CAS  Google Scholar 

  5. Rogers, P. L., Lee, K. J., and Tribe, D. E. (1979), Biotech. Lett. 1, 165–170.

    Article  CAS  Google Scholar 

  6. Lee, K. J. and Rogers, P. L. (1983), Chem. Eng. J. 27, B31–38.

    Article  CAS  Google Scholar 

  7. Lawford, H. G. and Stevnsborg, N. (1986), Biotechnol. Bioeng. Symp. 17, 209–219.

    CAS  Google Scholar 

  8. Lawford, H. G., Rousseau, J. D., and McMillan, J. D. (1997), Appl. Biochem. Biotechnol. 63/65, 269–286.

    Google Scholar 

  9. Warr, R. G., Goodman, A. E., Rogers, P. L., and Skotnicki, M. L. (1984) Microbios 41, 71–78.

    Google Scholar 

  10. Rogers, P. L. and Tribe, D. E. (1983), Ethanol Production. US Patent No. 4,403,034.

  11. Rogers, P.L. and Tribe, D.E. (1984), Semibatch Ethanol Production, US Patent No. 4,443,543.

  12. Rogers, P. L. and Tribe, D. E. (1984), Ethanol Production in a Continuous Process with Cell Recycle. US Patent No. 4,443,544.

  13. Lawford, H. G., Rousseau, J. D., Mohagheghi, A., and McMillan, J. D. (1998), Appl. Biochem. Biotechnol. 70/72, 353–367.

    Article  Google Scholar 

  14. Lawford, H. G. and Rousseau, J. D. (1999), Appl. Biochem. Biotechnol. 77–79, 235–249.

    Article  Google Scholar 

  15. Beyeler, W., Rogers, P. L., and Fiechter, A. (1984) Appl. Microbiol. Biotechnol. 19, 277–280.

    Article  CAS  Google Scholar 

  16. Rogers, P. L., Joachimsthal, E. L., and Haggett, K. D. (1997), Australasian Biotechnol. 7, 304–309.

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biotechnology, University of New South Wales, 2052, Sydney, Australia

    Eva Joachimsthal, Kevin D. Haggett & Peter L. Rogers

Authors
  1. Eva Joachimsthal
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kevin D. Haggett
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Peter L. Rogers
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Peter L. Rogers.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Joachimsthal, E., Haggett, K.D. & Rogers, P.L. Evaluation of recombinant strains of Zymomonas mobilis for ethanol production from glucose/xylose media. Appl Biochem Biotechnol 77, 147–157 (1999). https://doi.org/10.1385/ABAB:77:1-3:147

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1385/ABAB:77:1-3:147

Index Entries

Search

Navigation