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Enzyme recovery and recycling following hydrolysis of ammonia fiber explosion-treated corn stover

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Abstract

Both cellulase and cellobiase can be effectively recovered from hydrolyzed biomass using an ultrafiltration recovery method. Recovery of cellulase ranged from 60 to 66.6% and for cellobiase from 76.4 to 88%. Economic analysis shows that cost savings gained by enzyme recycling are sensitive to enzyme pricing and loading. At the demonstrated recovery of 60% and current loading of 15 Filter paper units of cellulase/g of glucan, enzyme recycling is expected to generate a cost savings of approx 15%. If recovery efficiency can be improved to 70%, the savings will increase to >25%, and at 90% recovery the savings will be 50%.

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Authors and Affiliations

  1. MBI International, 3900 Collins Road, 48910, Lansing, MI

    Bernie Steele, Srini Raj, John Nghiem & Mark Stowers

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  1. Bernie Steele
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  2. Srini Raj
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  3. John Nghiem
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  4. Mark Stowers
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Correspondence to Bernie Steele.

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Steele, B., Raj, S., Nghiem, J. et al. Enzyme recovery and recycling following hydrolysis of ammonia fiber explosion-treated corn stover. Appl Biochem Biotechnol 124, 901–910 (2005). https://doi.org/10.1385/ABAB:124:1-3:0901

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  • DOI: https://doi.org/10.1385/ABAB:124:1-3:0901

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