Abstract
Sawdust hydrolysates were investigated for their ability to support cell growth and cellulase production, and for potential inhibition of Trichoderma reesei Rut C30. Simultaneous fermentations were conducted to compare the hydrolysate-based media with the controls having equivalent concentrations of glucose and Avicel cellulose. Six hydrolysates differing in the boiling durations in the hydrolysis procedure were evaluated. The hydrolysates were found to support cell growth and induce active cellulase synthesis. The maximum specific cellulase production rate was 0.046 filter paper units (FPU)/(g of cells · h) in the hydrolysate-based systems, much higher than that (0.017 FPU/[g of cells · h]) in the controls.
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Lynd, L. R., Weimer, P. J., van Zyl, W. H., and Pretorius, I. H. (2002), Microbiol. Mol. Biol. Rev. 66, 506–577.
Araujo, A. and D’Souza, J. (1980), J. Ferment. Technol. 58, 399–401.
Mohagheghi, A., Grohmann, K., and Wyman, C. E. (1988), Appl. Biochem. Biotechnol. 17, 263–277.
Shin, C. S., Lee, J. P., Lee, J. S., and Park, S. C. (2000), Appl. Biochem. Biotechnol. 84–86, 237–245.
Ladisch, M. R., Hong, J., Voloch, M., and Tsao, G. T. (1981), in Trends in the Biology of Fermentation for Fuels and Chemicals, Hollaender, A., Rabson, R., Rodgers, P., San Pietro, A., Valentine, R., and Wolfe, R., eds., Plenum, New York, pp. 55–83.
Huang, A. A. (1975), Biotechnol. Bioeng. 17, 1421–1433.
Lee, Y. H. and Fan, L. T. (1982), Biotechnol. Bioeng. 24, 2383–2406.
Lee, Y. H. and Fan, L. T. (1983), Biotechnol. Bioeng. 25, 939–966.
Szengyel, Z., Zacchi, G., and Reczey, K. (1997), Appl. Biochem. Biotechnol. 63–65, 351–362.
Zhang, Q., Lo, C. M., and Ju, L.-K. (2004), submitted.
Sternberg, D. and Mandels, G. R. (1982), Exp. Mycol. 6, 115–124.
Jeong, J. M., Park, H. M., Hong, S. W., and Hah, Y. C. (1985), Misaengmul Hakhoechi 23, 77–83.
Mandels, M., Parrish, F. W., and Reese, E. T. (1962), J. Bacteriol. 83, 400–408.
Nisizawa, T., Suzuki, H., Nakayama, M., and Nisizawa, K. (1971), J. Biochem. (Tokyo) 70, 375–385.
Sternberg, D. and Mandels, G. R. (1979), J. Bacteriol. 139, 761–769.
Bailey, M. J. and Taehtiharju, J. (2003), Appl. Microbiol. Biotechnol. 62, 156–162.
Yasar, M., Akmaz, S., and Gurgey, I. (2001), Enzymatic hydrolysis of cellulose to glucose: Reaction kinetics and pathways. Abstracts of Papers, 222nd ACS National Meeting, BIOL-046, American Chemical Society, Washington, D.C.
Allen, A. L. and Mortensen, R. E. (1981), Biotechnol. Bioeng. 23, 2641–2645.
Ju, L.-K. and Afolabi, O. A. (1999), Biotechnol. Prog. 15, 91–97.
Lee, P. and Moore, M. (2002), Two-stage dilute acid hydrolysis of mixed southern hardwood: Process evaluation and optimization, Abstracts of Papers, 223rd ACS National Meeting, CELL-066, American Chemical Society, Washington, D.C.
McMillan, J. D. (1994), in Enzymatic conversion of biomass for fuels production, Michael E. Himmel, John O. Baker, Ralph P. Overend, eds., American Chemical Society, Washington, D.C.
Palmqvist, E., Hahn-Hagerdal, B., Szengyel, Z., Zacchi, G., and Reczey, K. (1997), Enzyme Microb. Technol. 20, 286–293.
Larsson, S., Palmqvist, E., Hahn-Hagerdal, B., Tengborg, C., Stenberg, K., Zacchi, G., and Nilvebrant, N.-O. (1999), Enzyme Microb. Technol. 24, 151–159.
Ranatunga, T. D., Jervis, J., Helm, R. F., McMillan, J. D., and Wooley, R. J. (2000), Enzyme Microb. Technol. 27, 240–247.
Larsson, S., Reimann, A., Nilvebrant, N.-O., and Jonsson, L. J. (1999), Appl. Biochem. Biotechnol. 77–79, 91–103.
Mandels, M., Andreotti, R., and Roche, C. (1976), Biotechnol. Bioeng. Symp. 6, 21–33.
Wu, J. and Ju, L.-K. (1998), Biotechnol. Prog. 14, 649–652.
Margollas-Clark, E., Hayes, C. K., Hatman, G. E., and Penttila, M. (1996), Appl. Environ. Microbiol. 62, 2145–2151.
McMillan, J. D. (1994), in Enzymatic conversion of biomass for fuels production, Michael E. Himmel, John O. Baker, Ralph P. Overend, eds., American Chemical Society, Washington, D.C.
Marten, M. R., Velkovska, S., Khan, S. A., and Ollis, D. F. (1996), Biotechnol. Prog. 12, 602–611.
Schaffner, D. W. and Toledo, R. T. (1991), Biotechnol. Bioeng. 37, 12–16.
Tao, H., Gonzalez, R., Martinez, A., Rodriguez, M., Ingram, L. O., Preston, J. F., and Shanmugam, K. T. (2001), J. Bacteriol. 183, 2979–2988.
Seiboth, B., Hartl, L., Pail, M., and Kubicek, C. P. (2003), Eukaryotic Cell 2, 867–875.
Sanchez, S., Bravo, V., Castro, E., Moya, A. J., and Camacho, F. (2002), J. Chem. Technol. Biotechnol. 77, 641–648.
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Lo, CM., Zhang, Q., Lee, P. et al. Cellulase production by Trichoderma reesei using sawdust hydrolysate. Appl Biochem Biotechnol 122, 561–573 (2005). https://doi.org/10.1385/ABAB:122:1-3:0561
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DOI: https://doi.org/10.1385/ABAB:122:1-3:0561