Abstract
S-layer protein of Bacillus thuringiensis strain CTC was used as the carrier protein to display polyhistidine (poly[6His]) peptides on the cell surface. Poly(6His) n was fused with S-layer protein at two different sites, inserting just downstream of the S-layer protein homologous domain (slh) and replacing the non-slh region of S-layer protein, respectively. The two series chimeric proteins were both expressed by crystal negative B. thuringiensis strain 4Q7 and strain 171, respectively, as shown by sodium dodecyl sulfate polyacrylamide gel electrophoresis. The recombinant B. thuringiensis cells gained Ni2+- and Cd2+-binding ability and had a capacity to display up to nine copies of poly(6His). The Cd2+ adsorption quantity of the recombinant strain with the strongest adsorption ability was twice that of the host strain.
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Wang, L., Sun, M. & Yu, Z. Capacity of Bacillus thuringiensis S-layer protein displaying polyhistidine peptides on the cell surface. Appl Biochem Biotechnol 119, 133–143 (2004). https://doi.org/10.1385/ABAB:119:2:133
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DOI: https://doi.org/10.1385/ABAB:119:2:133