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Kinetics of asparaginase II fermentation in Saccharomyces cerevisiae ure2dal80 mutant

Effect of nitrogen nutrition and pH

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Abstract

Although the quality of nitrogen source affects fermentation product formation, it has been managed empirically, to a large extent, in industrial scale. Laboratory-scale experiments successfully use the high-cost proline as a nonrepressive source. We evaluated urea as a substitute for proline in Saccharomyces cerevisiae ure2dal80 fermentations for asparaginase II production as a model system for nitrogen-regulated external enzymes. Maximum asparaginase II levels of 265 IU/L were observed in early stationary-phase cells grown on either proline or urea, whereas in ammonium cells, the maximum enzyme level was 157 IU/L. In all cases, enzyme stability was higher in buffered cultures with an initial pH of 6.5.

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Authors and Affiliations

  1. Far-Manguinhos, FIOCRUZ, Av. Sizenando Nabuco, 100, 21041-250, Rio de Janeiro, RJ, Brazil

    Maria Antonieta Ferrara

  2. Departamento de Bioquímica, Instituto de Química, Universidade Federal do Rio de Janeiro, RJ 22949-900, Brazil

    Josiane M. V. Mattoso & Elba P. S. Bon

  3. Escola de Química, Universidade Federal do Rio de Janeiro, CT Bloco E, 22945-900, Rio de Janeiro, RJ, Brazil

    Nei Pereira Jr.

Authors
  1. Maria Antonieta Ferrara
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  2. Josiane M. V. Mattoso
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  3. Elba P. S. Bon
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  4. Nei Pereira Jr.
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Correspondence to Maria Antonieta Ferrara.

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Ferrara, M.A., Mattoso, J.M.V., Bon, E.P.S. et al. Kinetics of asparaginase II fermentation in Saccharomyces cerevisiae ure2dal80 mutant. Appl Biochem Biotechnol 113, 299–305 (2004). https://doi.org/10.1385/ABAB:113:1-3:299

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  • DOI: https://doi.org/10.1385/ABAB:113:1-3:299

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