Abstract
The production of lipases by Penicillium simplicissimum using solid-state fermentation and soy cake as substrate was investigated. The effects of temperature, cake moisture, and carbon supplementation on lipase production were studied using a two-level experimental plan. Moisture, pH, and lipase activity were followed during fermentation. Statistical analysis of the results was performed to evaluate the effect of the studied variables on the maximum lipase activity. Incubation temperature was the variable that most affected enzyme activity, showing a negative effect. Moisture and carbon supplementation presented a positive effect on activity. It was possible to obtain lipase activity as high as 21 U/g of dry cake in the studied range of process variables.
Similar content being viewed by others
References
Jung, F., Cammarota, M. C., and Freire, D. M. G. (2002), Biotechnol. Lett. 24, 1797–1802.
Cammarota, M. C., Teixeira, G. A., and Freire, D. M. G. (2001), Biotechnol. Lett. 23, 1591–1595.
Castilho, L. R., Polato, C. M. S., Baruque, E. A., and Sant’Anna, G. L., Jr. (2000), Biochem. Eng. J. 4, 239–247.
Del Bianchi, V. L., Moraes, I. O. and Capalbo, D. M. F. (2001), in Biotecnologia Industrial: Engenharia Bioquímica, vol. 3, Schmidell, W., Lima, U.A., Aquarone, E., and Borzani, W., eds., Edgard Blücher, São Paulo, Brazil.
Freire, D. M. G. (1996), PhD thesis, PEQ/COPPE/UFRJ, Rio de Janeiro, Brazil.
Freire, D. M. G., Gomes, P. M., Bon, E. P. S., and Sant’Anna, G. L., Jr. (1997), Revista Microbiologia 28(1), 6–12.
Leal, M. C. M. R. (2000), MS thesis, PEQ/COPPE/UFRJ, Rio de Janeiro, Brazil.
Pregnolatto, W., Pregnolatto, N. P. (coord.), and Rebocho, D. D. E. (ed.) (1985), Normas analíticas do Instituto Adolfo Lutz: vol. 1. Métodos químicos e físicios para análise de alimentos, 3rd Ed., Instituto Adolfo Lutz, São Paulo, Brazil.
MCT, Ministério da Ciênciae Tecnologia (Ministry of Science and Technology) (1985), Technical Report on Vegetable Oils, MCT.
Gombert, A. K., Pinto, A. L., Castilho, L. R., and Freire, D. M. G. (1999), Process Biochem. 35, 85–90.
Palma, M. B., Pinto, A. L., Gombert, A. K., Seitz, K. H., Kivatinitz, S. C., Castilho, L. R., and Freire, D. M. G. (2000), Appl. Biochem. Biotechnol. 84–86, 1137–1145.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
di Luccio, M., Capra, F., Ribeiro, N.P. et al. Effect of temperature, moisture, and carbon supplementation on lipase production by solid-state fermentation of soy cake by Penicillium simplicissimum . Appl Biochem Biotechnol 113, 173–180 (2004). https://doi.org/10.1385/ABAB:113:1-3:173
Issue Date:
DOI: https://doi.org/10.1385/ABAB:113:1-3:173