Abstract
EDTA, the target compound of this study from the effluent of secondary biotreatment units, can be biodegraded by special microorganisms. So far, there are three species of microorganisms—Agrobacterium, Gram-negative BNCI, and DSM9103—that can degrade EDTA and are published in the literature. We have successfully isolated a bacterial strain that can degrade EDTA. It was identified as Burkhol cepacia, an aerobic species, elliptically shaped with a length of 5–15 μm. The growth medium contains 1000 mg/L of ferric-EDTA as carbon source, 750 mg/L of (NH4)2SO4+(NH2)2CO as nitrogen source, and 1000 mg/L of KH2PO4 as phosphorus source, and mineral factors such as Fe and Mg. Incubated at pH, 7.0, 30°C, and 150 rpm on a shaker for 15 d, the average specific growth rate of this microbe is 0.135 d−1, which shows that the respective degradation efficiency of Fe-EDTA and Cu-EDTA is 90 and 75% individually.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Witschel, M., Nagel, S., and Egli, T. (1997), J. Bacteriol. 179(22), 6937–6943.
Kari, F. G. and Giger, W. (1996), Water Res. 30(1), 122–134.
Payne, J. W., Bolton, H., Jr., Campbell, C., and Xum, L. (1998), J. Bacteriol. 180(15), 3823–3827.
Dietz, F. (1987), Wasser-GWF. 128, 286–288.
Gardiner, J. (1976), Water Res. 10, 507–514.
Van Dijk-Looijaard, A. M., De Groot, A. C., Janssen, P. J. C. M., and Wondergem, E. A. (1990), RIVM report no 718629006, Rijksinstitut voor Voksgesondheid en Miliue, Bilthoven, The Netherlands.
Van Dijk-Looijaard, A. M., De Groot, A. C., Janssed, P. J. C. M., and Wondergem, E. A. (1990), H2O 33, 682–687.
Van Ginkel, C. G., Vandenbroucke, K. L., and Stroo, C. A. (1997), Bioresour. Technol. 59, 151–155.
Egli, T. (1988), Microb. Sci. 5, 36–41.
Egli, T., Weilenman, H. U., El-Banna, T., and Auling, G. (1988), Syst. Appl. Microbiol. 10, 297–305.
Van Ginkel, C. G., Kester, H., Stroo, C. A., and van Haperen, A. M. (1999), Water Sci. Technol. 40(11–12), 259–265.
Thomas Russell, A. P. et al. (1998), Appl. Environ. Microbiol. 4, 1319–1322.
Bolton, H. Jr., Li, S. W., Workman, D. J., and Girvin, D. C. (1993), J. Environ. Quality 22, 125–132.
Tiedje, J. M. (1975), Appl. Microbiol. 30, 327–329.
Palumbo, A. V., Lee, S. Y., and Boerman, P. (1994), Appl. Biochem. Biotechnol. 45, 811–822.
Lauff, J. J., Steele, D. B., Coogan, L. A., and Breitfeller, J. M. (1990), Appl. Environ. Microbiol. 56(11), 3346–3353.
Witschel, M., Egli, T., Zehnder, A. J. B., Wehrli, E., and Spycher, M. (1999), Microbiology 145, 973–983.
Virtapohja, J. and Alen, R. (1999), Chemosphere 38(1), 143–154.
APHA-AWWA-WPCF. (1992), Standard Methods for the Examination of Water and Wastewater, 18th ed. Washington DC, American Public Health Association, American Water Works Association and Water Pollution Control Federation.
Belly, R. T., Lauff, J. J., and Goodhue, C. T. (1975), Appl. Microbiol. 29, 787–794.
Lauff, J. J., Steele, D. B., Coogan, L. A., and Breitfeller, J. M. (1990), Appl. Environ. Microbiol. 56, 3346–3353.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Fang, HY., Chen, SC. & Chen, SL. Degradation of ferric EDTA by Burkhol cepacia . Appl Biochem Biotechnol 111, 81–91 (2003). https://doi.org/10.1385/ABAB:111:2:81
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1385/ABAB:111:2:81