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Detecting mycotoxins in agricultural commodities

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Abstract

It is difficult to obtain precise and accurate estimates of the true mycotoxin concentration of a bulk lot when using a mycotoxin-sampling plan that measures the concentration in a small portion of the bulk lot. A mycotoxin-sampling plan is defined by a mycotoxin test procedure and a defined accept/reject limit. A mycotoxin test procedure is a complicated process and generally consists of several steps: (a) a sample is taken from the lot, (b) the sample is ground (comminuted) in a mill to reduce particle size, (c) a subsample is removed from the comminuted sample, and (d) the mycotoxin is extracted from the comminuted subsample and quantified. Even when using accepted test procedures, there is variability associated with each step of the mycotoxin test procedure. Because of this variability, the true mycotoxin concentration in the lot cannot be determined with 100% certainty by measuring the mycotoxin concentration in a sample taken from the lot. The variability for each step of the mycotoxin test procedure, as measured by the variance statistic, is shown to increase with mycotoxin concentration. Sampling is usually the largest source of variability associated with the mycotoxin test procedure. Sampling variability is large because a small percentage of kernels are contaminated and the level of contamination on a single seed can be very large. Methods to reduce sampling, sample preparation, and analytical variability are discussed.

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References

  1. Whitaker, T. B. and Dowell, F. E. (1995) Sampling methods to measure aflatoxin and grade factors of peanuts. In Advances in Peanut Science. Pattee, H. E. and Stalker, H. T., eds., Am. Peanut Res. Educ. Soc., Stillwater, OK., pp 475–499.

    Google Scholar 

  2. Association of Official Analytical Chemists (1990) Official Methods of Analysis of the Association of Official Analytical Chemists, (Kenneth Helrich, ed.), 15th ed., Arlington, Va., 20877–22417.

  3. Nesheim, S. (1979) Methods of Aflatoxin Analysis, NBS Spec. Publ., (US) No.519, pp. 355.

  4. Schuller, P. O., Horwitz, W., and Stoloff, L. (1976) A review of sampling and collaboratively studied methods of analysis for aflatoxin. J. Assoc. Off. Anal. Chem, Int. 59, 1315.

    CAS  Google Scholar 

  5. Campbell, A. D., Whitaker, T. B., Pohland, A. E., Dickens, J. W., and Park, D. L. (1986) Sampling, sample preparation, and sampling plans for foodstuffs for mycotoxin analysis. Pure and Applied Chem. 58, 305–314.

    CAS  Google Scholar 

  6. Food and Agriculture Organization (1993) Sampling Plans for Aflatoxin Analysis in Peanuts and Corn. FAO Food and Nutrition Paper 55. FAO, Viale della Terme di Caracalla, 00100, Rome, Italy.

  7. Park, D. L. and Pohland, A. E. (1989) Sampling and sample preparation for detection of natural toxicants in food and feed. J. Assoc. Off. Anal. Chem., Int. 72, 399–404.

    CAS  Google Scholar 

  8. Whitaker, T. B., Dickens, J. W., and Monroe, R. J. (1974) Variability of aflatoxin test results, J. Am. Oil Chem. Soc. 51, 214–218.

    Article  PubMed  CAS  Google Scholar 

  9. Whitaker, T. B., Whitten, M. E., and Monroe, R. J. (1976) Variability associated with testing cottonseed for aflatoxin. J. Am. Oil Chem. Soc. 53, 502–505.

    Article  PubMed  CAS  Google Scholar 

  10. Whitaker, T. B., Dickens, J. W., and Monroe, R. J. (1979) Variability associated with testing corn for aflatoxin. J. Am. Oil Chem. Soc. 56, 789–794.

    CAS  Google Scholar 

  11. Whitaker, T. B., Dowell, F. E., Hagler, W. M., Jr., Giesbrecht, F. G., and Wu, J. (1993) Variability associated with sampling, sample preparation, and chemically testing farmers stock peanuts for aflatoxin. J. Assoc. Off. Anal. Chem., Int. 77, 107–116.

    Google Scholar 

  12. Schatzki, T. F. (1995) Distribution of aflatoxin in pistachios. 1. Lot distributions. J. Agric. Food. Chem. 43, 1561–1565.

    Article  CAS  Google Scholar 

  13. Schatzki, T. F. (1995) Distribution of aflatoxin in pistachios. 2. Distribution in freshly harvested pistachios. J. Agric. Food. Chem. 43, 1566–1569.

    Article  CAS  Google Scholar 

  14. Whitaker, T. B., Truckess, M. W., Johansson, A. S., Giesbrecht, F. G., Hagler, W. M., Jr., and Bowman, D. T. (1998) Variability associated with testing shelled corn for fumonisin. J. Assoc. Off. Anal. Chem., Int. 81, 1162–1168.

    CAS  Google Scholar 

  15. Hart, L. P. and Schabenberger, O. (1998) Variability of vomitoxin in truckloads of wheat in a wheat scab epidemic year. Plant Disease 82, 625–630.

    Article  Google Scholar 

  16. Whitaker, T. B., Dickens, J. W., and Monroe, R. J. (1972) Comparison of the observed distribution of aflatoxin in shelled peanuts to the negative binomial distribution. J. Am. Oil Chem. Soc. 49, 590–593.

    Article  PubMed  CAS  Google Scholar 

  17. Dickens, J. W. and Satterwhite, J. B. (1969) Subsampling mill for peanut kernels. Food Tech. 23, 90–92.

    Google Scholar 

  18. Remington, R. D. and Schrok, M. A. (1970) Statistics and Applications to the Biological and Health Sciences. Prentice-Hall, Englewood Cliffs, NJ, pp. 105.

    Google Scholar 

  19. Hart, L. P. and Schabenberger, O. (1998) Variability of vomitoxin in truckloads of wheat in a wheat scab epidemic year. Plant Disease 82, 625–630.

    Article  Google Scholar 

  20. Johansson, A. S., Whitaker, T. B., Hagler, W. M., Jr., Giesbrecht, F. G., Young, J. H., and Bowman, D. T. (2000) Testing shelled corn for aflatoxin, Part I: Estimation of variance components. J. Assoc. Off. Anal. Chem., Int. 83, 1264–1269.

    CAS  Google Scholar 

  21. Whitaker, T. B., Hagler, W. M. Jr., Giesbrecht, F. G., and Johansson, A. S. (2000) Sampling, sample preparation, and analytical variability associated with testing wheat for deoxynivalenol. J. Assoc. Off. Anal. Chem., Int. 83, 1285–1292.

    CAS  Google Scholar 

  22. Whitaker, T. B. and Wiser, E. H. (1969) Theoretical investigations into the accuracy of sampling shelled peanuts for aflatoxin. J. Am. Oil Chem. Soc. 46, 377–379.

    Article  PubMed  CAS  Google Scholar 

  23. Johansson, A. S., Whitaker, T.B., Hagler, W. M., Jr., Giesbrecht, F. G., and Young, J.H. (2000) Testing shelled corn for aflatoxin, Part II: Modeling the distribution of aflatoxin test results. J. Assoc. Off. Anal. Chem., Int. 83, 1270–1278.

    CAS  Google Scholar 

  24. Cucullu, A. F., Lee, L. S., Mayne, R. Y., and Goldblatt, L. A. (1986) Determination of aflatoxin in individual peanuts and peanut sections. J. Am. Oil Chem. Soc. 43, 89.

    Article  Google Scholar 

  25. Cucullu, A. F., Lee, L. S., and Pons, W. A. (1977) Relationship of physical appearance of individual mold damaged cottonseed to aflatoxin content. J. Am. Oil Chem. Soc. 54, 235A.

    Google Scholar 

  26. Shotwell, O. L., Goulden, M. L., and Hessletine, C. W. (1974) Aflatoxin: Distribution in contaminated corn. Cereal Chem. 51, 492.

    CAS  Google Scholar 

  27. Pohland, A. E. and Trucksess, M. W. (2000) Mycotoxin method evaluation. In Methods in Molecular Biology, vol. 157: Mycotoxin Protocols. Trucksess, M. W. and Pohland, A. E., eds., Humana Press, Totowa, NJ, pp. 3–10.

    Google Scholar 

  28. Whitaker, T. B., Horwitz, W., Albert, R., and Nesheim, S. (1996) Variability associated with methods used to measure aflatoxin in agricultural commodities. J. Assoc. Off. Anal. Chem., Int. 79, 476–485.

    CAS  Google Scholar 

  29. Whitaker, T. B. and Dickens, J. W. (1979) Evaluation of the Peanut Administrative Committee testing program for aflatoxin in shelled peanuts. Peanut Sci. 6, 7–9.

    Article  Google Scholar 

  30. Johansson, A. S., Whitaker, T. B., Hagler, W. M., Jr., Giesbrecht, F. G., and Young, J. H. (2000) Testing shelled corn for aflatoxin, Part III: Evaluating the performance of aflatoxin sampling plans. J. Assoc. Off. Anal. Chem., Int. 83, 1279–1284.

    CAS  Google Scholar 

  31. Whitaker, T. B., Springer, J., Defize, P. R., deKoe, W. J., and Coker, R. (1995) Evaluation of sampling plans used in the United States, United Kingdom, and The Netherlands to test raw shelled peanuts for aflatoxin. J. Assoc. Off. Anal. Chem., Int. 78, 1010–1018.

    CAS  Google Scholar 

  32. Whitaker, T. B. and Dickens, J. W. (1989) Simulating aflatoxin testing plans for shelled peanuts in the export market. J. Assoc. Off. Anal. Chem., Int. 72, 644–648.

    CAS  Google Scholar 

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  1. USDA/ARS, North Carolina State University, Box 7625, 27695-7625, Raleigh, NC

    Thomas B. Whitaker

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Whitaker, T.B. Detecting mycotoxins in agricultural commodities. Mol Biotechnol 23, 61–71 (2003). https://doi.org/10.1385/MB:23:1:61

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