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Chromatographia

, Volume 72, Issue 9–10, pp 815–819 | Cite as

An Improved Method for the Separation and Quantification of Major Phospholipid Classes by LC-ELSD

  • Kun-Ping Yan
  • Hong-Li Zhu
  • Ning Dan
  • Chao Chen
Original

Abstract

An improved HPLC procedure for the separation of phospholipids is described. The method described utilizes a solvent mixture of acetonitrile-methanol–water-trifluoroacetic acid (100:25:1.7:2.5, v/v) as the mobile phase, which is more compatible with the pump than mobile phases containing inorganic acids. Separation was by isocratic elution on a Hypersil silica column coupled to an evaporative light scattering detector. Complete separation of phosphatidylserine (PS), phosphatidylethanolamine (PE), phosphatidylcholine (PC) and sphingomyelin (SM) was achieved in less than 20 min. The detection limits for PS, PE, PC and SM were 50, 50, 80 and 150 ng (S/N = 3), respectively. Human, bovine and porcine erythrocyte ghost membranes and animal tissues have been successfully analyzed for their phospholipid contents.

Keywords

Column liquid chromatography Evaporative light scattering detector Phospholipids Trifluoroacetic acid 

Notes

Acknowledgments

The study was supported by grants from the National 863 Program of China (Grants No. 2006AA02A143) and “Special Research Foundation” (Grants No. 09JK784 and 08JK469) by Education Department of Shaanxi Province.

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Copyright information

© Vieweg+Teubner Verlag | Springer Fachmedien Wiesbaden GmbH 2010

Authors and Affiliations

  • Kun-Ping Yan
    • 1
  • Hong-Li Zhu
    • 1
    • 2
  • Ning Dan
    • 1
    • 2
  • Chao Chen
    • 1
    • 3
  1. 1.College of Life Science, Northwest UniversityXi’anPeople’s Republic of China
  2. 2.Shaanxi Lifegen Co., LtdXi’anPeople’s Republic of China
  3. 3.National Engineering Research Center for Miniaturized Detection SystemXi’anPeople’s Republic of China

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