A GC–MS-SIM Simultaneous Determination of Ligustilide and Butylidenephthalide from Ligusticum chuanxiong Using SFE
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A simple capillary GC–MS method for the simultaneous determination of ligustilide (LGT) and butylidenephthalide (BDP) in Ligusticum chuanxiong is described. LGT and BDP were obtained by supercritical fluid extraction (SFE), and muscone was used in this method as an internal standard for quantification. MS detection was performed in SIM mode to increase the sensitivity. The tested ranges were 20–1,000 μg mL−1 for LGT and 2–100 μg mL−1 for BDP. This method was fully validated and showed good performances in terms of recovery (98.2–101.5%) and precision (1.21–3.52%). Finally, the method was applied to the analysis of LGT and BDP in L. chuanxiong by SFE.
KeywordsGas chromatography–mass spectrometry Supercritical fluid extraction Butylidenephthalide Ligustilide Linusticumchuanxiong
These works were supported by the Hubei Education Department Science and Technology Foundation of China (Nos.D20102108 and B20092410) and the Hubei National Scientific Foundation of China (No.2009CDB083).
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