, Volume 72, Issue 9–10, pp 999–1004 | Cite as

Determination of Diacetyl in Butter and Air Samples by SPME Coupled with GC–MS

  • Yong Chen
  • Robert E. Shirey
  • Leonard M. Sidisky
Full Short Communication


A solid phase microextraction-gas chromatography-mass spectrometry method was developed to determine diacetyl in butter samples. The extraction was performed for 5 min at 37 °C using a polydimethylsiloxane/divinyl benzene fiber. The method had a linear range of 0.024–11.2 ppm with limit of detection of 0.0078 ppm. Air sampling of diacetyl using solid phase microextraction was also developed to estimate diacetyl concentration in air. The extraction was performed by exposure of a polydimethylsiloxane fiber to air for 2 min. This method had linear range of 0.2–22 ppm (v/v) with limit of detection of 0.05 ppm.


Gas chromatography–mass spectrometry Solid phase microextraction Diacetyl Isotope dilution 



The authors would like to thank Jamie Brown for providing diacetyl standards and popcorn samples.


  1. 1.
    Mallia S, Escher F, Schlichtherle-Cerny H (2008) Eur Food Res Technol 226:315–325CrossRefGoogle Scholar
  2. 2.
    Monnet C, Schmitt P, Divies C (1994) J Diary Sci 77:1809–1815CrossRefGoogle Scholar
  3. 3.
    Kolb B, Ettre LS (2006) Static headspace-gas chromatography theory and practice, 2nd edn. Wiley, HobokenGoogle Scholar
  4. 4.
    Macciola V, Candela G, De Leonardis A (2008) Food Control 19:873–878CrossRefGoogle Scholar
  5. 5.
    Guerra Hernández EJ, Garćia Estepa R, Rodriguez Rivas I (1995) Food Chem 53:315–319CrossRefGoogle Scholar
  6. 6.
    Matsuura H, Fujiyama K, Minagawa N, Sawa J (1990) Bunseki Kagaku 39:405–409Google Scholar
  7. 7.
    Zeppa G, Conterno L, Gerbi G (2001) J Agric Food Chem 49:2722–2726CrossRefGoogle Scholar
  8. 8.
    Schieberle P, Gassenmeier K, Guth H, Sen A, Grosch W (1993) Lebensm Wiss Technol 26:347–356CrossRefGoogle Scholar
  9. 9.
    Pawliszyn J (1997) Solid phase microextraction—theory and practice. Wiley, New YorkGoogle Scholar
  10. 10.
    Berlardi RG, Pawliszyn J (1989) Water Pollut Res J Can 24:179–191Google Scholar
  11. 11.
    Arthur CL, Pawliszyn J (1990) Anal Chem 62:2145–2148CrossRefGoogle Scholar
  12. 12.
    Pawliszyn J (1999) (ed) Applications of solid phase microextraction. RSC, CambridgeGoogle Scholar
  13. 13.
    Scheppers-Wercinski SA (1999) (ed) Solid phase microextraction: a practical guide. Dekker, New YorkGoogle Scholar
  14. 14.
    Pawliszyn J (2002) (ed) Sampling and sample preparation for field and laboratory. Elsevier, Amsterdam, The NetherlandsGoogle Scholar
  15. 15.
    Arthur CL, Killam LM, Buchholz KD, Pawliszyn J, Berg JR (1992) Anal Chem 64:1960–1966CrossRefGoogle Scholar
  16. 16.
    O’Reilly J, Wang Q, Setkova L, Hutchinson JP, Chen Y, Lord HL, Linton CM, Pawliszyn J (2005) J Sep Sci 28:2010–2022CrossRefGoogle Scholar
  17. 17.
    Jia M, Koziel J, Pawliszyn J (2000) Field Anal Chem Technol 4:73–84CrossRefGoogle Scholar
  18. 18.
    Augusto F, Koziel J, Pawliszyn J (2001) Anal Chem 73:481–486CrossRefGoogle Scholar
  19. 19.
    Povolo M, Contarini G (2003) J Chromatogr A 985:117–125CrossRefGoogle Scholar
  20. 20.
    Belitz HD, Grosch W, Schieberle P, (2009) (eds) Food chemistry, 4th edn. Springer, Heidelberg, Germany, pp 524Google Scholar
  21. 21.
    Zhang Z, Pawliszyn J (1993) Anal Chem 65:1843–1852CrossRefGoogle Scholar
  22. 22.
    Chen Y, Begnaud F, Chaintreau A, Pawliszyn J (2006) Flavor Frag J 21:822–832CrossRefGoogle Scholar
  23. 23.
    Marangoni AG, Lencki RW (1998) J Agric Food Chem 46:3879–3884CrossRefGoogle Scholar
  24. 24.
    Milo C, Blank I (1998) Quantification of impact ordorants in food by isotope dilution assay: strength and limitations. In: Mussinan CJ, Morello MJ (eds) Flavor analysis: developments in isolation and characterization, ACS Symposium Series, American Chemical Society, Washington, 705:250–259Google Scholar
  25. 25.
    Arctander S (1994) (ed) Perfume and flavor chemicals (Aroma Chemicals) vol 1. Allured Publishing, Carol StreamGoogle Scholar

Copyright information

© Vieweg+Teubner Verlag | Springer Fachmedien Wiesbaden GmbH 2010

Authors and Affiliations

  • Yong Chen
    • 1
  • Robert E. Shirey
    • 1
  • Leonard M. Sidisky
    • 1
  1. 1.SupelcoBellefonteUSA

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