, Volume 72, Issue 7–8, pp 731–735 | Cite as

Determination of Azithromycin in Biological Samples by LLLME Combined with LC

  • Homeira Ebrahimzadeh
  • Yadollah Yamini
  • Katayoun Mahdavi Ara
  • Fahimeh Kamarei
  • Farahnaz Khalighi-Sigaroodi
Full Short Communication


A simple liquid–liquid–liquid microextraction (LLLME) technique combined with liquid chromatography (LC) was developed for the extraction and quantitative determination of azithromycin (AZI) in biological fluids. In this technique, AZI was extracted from 2.5 mL basic sample solution (donor phase) at pH 10.5 into an organic phase (90 μL), for 30 min. Then back-extracted into a 5 μL microdrop acidic aqueous solution (acceptor phase) at pH 2.5 immersed in the organic phase from the tip of a microsyringe for 20 min with a stirring rate of 800 rpm and donor phase temperature of 35 °C. After a prescribed time, the acceptor microdrop was returned into the microsyringe and injected into the LC. Optimization of experimental parameters on LLLME efficiency was investigated. Under optimized conditions, a preconcentration factor of 85 and limit of detection of 0.03 μg mL−1 were obtained. The calibration curve was linear (r 2 = 0.998) in the concentration range of 0.1–15 μg mL−1. Within-day relative standard deviation (RSD) (S/N = 3) and between-day RSD were 5.1 and 6.8%, respectively. Finally, the feasibility of the proposed method was evaluated by extraction and determination of AZI in plasma and urine samples and satisfactory results were obtained.


Column liquid chromatography Liquid–liquid–liquid microextraction Azithromycin in plasma and urine 


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Copyright information

© Vieweg+Teubner Verlag | Springer Fachmedien Wiesbaden GmbH 2010

Authors and Affiliations

  • Homeira Ebrahimzadeh
    • 1
  • Yadollah Yamini
    • 2
  • Katayoun Mahdavi Ara
    • 1
  • Fahimeh Kamarei
    • 1
  • Farahnaz Khalighi-Sigaroodi
    • 3
  1. 1.Department of ChemistryShahid Beheshti UniversityTehranIran
  2. 2.Department of ChemistryTarbiat Modares UniversityTehranIran
  3. 3.Department of Pharmacognosy and PharmaceuticsInstitute of Medicinal Plants, ACECRKarajIran

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