Abstract
A simple liquid–liquid–liquid microextraction (LLLME) technique combined with liquid chromatography (LC) was developed for the extraction and quantitative determination of azithromycin (AZI) in biological fluids. In this technique, AZI was extracted from 2.5 mL basic sample solution (donor phase) at pH 10.5 into an organic phase (90 μL), for 30 min. Then back-extracted into a 5 μL microdrop acidic aqueous solution (acceptor phase) at pH 2.5 immersed in the organic phase from the tip of a microsyringe for 20 min with a stirring rate of 800 rpm and donor phase temperature of 35 °C. After a prescribed time, the acceptor microdrop was returned into the microsyringe and injected into the LC. Optimization of experimental parameters on LLLME efficiency was investigated. Under optimized conditions, a preconcentration factor of 85 and limit of detection of 0.03 μg mL−1 were obtained. The calibration curve was linear (r 2 = 0.998) in the concentration range of 0.1–15 μg mL−1. Within-day relative standard deviation (RSD) (S/N = 3) and between-day RSD were 5.1 and 6.8%, respectively. Finally, the feasibility of the proposed method was evaluated by extraction and determination of AZI in plasma and urine samples and satisfactory results were obtained.
References
Shaikh KA, Patil SD, Devkhile AB (2008) J Pharm Biomed Anal 48:1481–1484
Moffat AC (ed) (1986) Clarke’s isolation and identification of drugs. The Pharmaceutical Press, London
Koch DE, Bhandari A, Closb L, Hunter RP (2005) J Chromatogr A 1074:17–22
Bahrami G, Mirzaeei S, Kiani A (2005) J Chromatogr B 820:277–281
Chen BM, Liang YZ, Chen X, Liu SG, Deng FL, Zhou P (2006) J Pharm Biomed Anal 42:480–487
Chen L, Qin F, Ma Y, Li F (2007) J Chromatogr B 855:255–261
Nirogi RVS, Kandikere VN, Shukla M, Mudigonda K, Maurya S, Boosi R, Yerramilli A (2005) Anal Chem Acta 553:1–8
Prosen H, Zupancic-Kralj L (1999) Trends Anal Chem 18:272–282
Shariati S, Yamini Y, Darabi M, Amini M (2007) J Chromatogr B 855:228–235
Ebrahimzadeh H, Yamini Y, Sedighi A, Rouini MR (2008) J Chromatogr B 863:229–234
Ebrahimzadeh H, Yamini Y, Gholizade A, Sedighi A, Kasraee S (2008) Anal Chem Acta 626:193–199
Psillakis E, Kalogerakis N (2003) Trends Anal Chem 22:565–574
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Ebrahimzadeh, H., Yamini, Y., Ara, K.M. et al. Determination of Azithromycin in Biological Samples by LLLME Combined with LC. Chroma 72, 731–735 (2010). https://doi.org/10.1365/s10337-010-1692-9
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DOI: https://doi.org/10.1365/s10337-010-1692-9